脱脂麦胚水提液中谷胱甘肽的初步分离

Isolation of Glutathione from Aqueous Extract of Defatted Wheat Germ

对脱脂麦胚水提液中的谷胱甘肽的分离进行研究。通过对谷胱甘肽的稳定性进行研究以及比较不同纯化工艺组合对谷胱甘肽质量分数的影响,建立采用超滤结合离子交换树脂层析分离脱脂麦胚水提液中谷胱甘肽的工艺技术,结果所得产品中谷胱甘肽的质量分数由未纯化前的0.79%提高到8.8%,为进一步纯化谷胱甘肽提供了良好的方法。

The isolation of glutathione（GSH） from aqueous extract of defatted wheat germ（DWG） was explored.The effects of temperature and pH on the stability of GSH in aqueous extract of DWG were studied during.An isolation method for isolating GSH from aqueous extract of DWG was established using ultra-filtration followed by TOSO SP-650M ion-exchange resin chromatography.The GSH content in the isolated product was 8.8% compared with 0.79% in aqueous extract of DWG.