马兰头多糖提取工艺的响应面法优化及不同部位多糖含量的测定

Optimization of Extraction Process for Polysaccharides from Kalimeris indica L. by Response Surface Methodology and Polysaccharide Contents in Different Parts

以连云港花果山野生马兰头地上部分为原料,研究马兰头多糖的提取工艺。在单因素试验基础上,根据Box-Behnken试验设计原理,选取液料比、提取时间和提取温度三因素三水平进行中心组合试验,通过响应面分析得到优化组合条件。结果表明提取工艺最佳条件为提取温度98℃、提取时间3.5h、液料比33:1(mL/g)、提取2次。该条件下多糖提取率预测值为4.2037%,验证值为4.1946%。对马兰头不同部位多糖含量测定的结果表明,马兰头不同部位多糖含量存在明显差异,叶中最高,为4.9407%,茎次之,为1.5356%,根最低,仅有0.6817%。

Polysaccharide extraction from the aboveground parts of Kalimeris indica L.wildly grown in the Huaguoshan Mountain Area of Lianyungan was studied.One-factor-at-a-time method was used to identify extraction parameters such as material-to-liquid ratio,time and temperature as main factors that influence the extraction yield of polysaccharides.A 3-factor,3-level central composite design involving the three independent variables（extraction parameters） followed by response surface analysis was carried out to optimize the extraction parameters.The optimal extraction conditions were twice repeated extractions at 98 ℃ and a material-to-liquid ratio of 33：1（mL/g） for 3.5 h each time.Under these conditions,the predicted and experimental extraction yields of polysaccharides were 4.2037% and 4.1946%,respectively.The results of determination of polysaccharide content showed that significant differences in polysaccharide content existed among different parts of the plant： the highest polysaccharide content,4.9407%,was observed in the leaves followed by the stems,1.5356%,and the roots exhibited the lowest polysaccharide content,only 0.6817%.