摘要
为筛选拮抗胡麻枯萎病菌的生防微生物,对采自我国不同省份胡麻根围土壤进行了细菌分离和拮抗菌筛选。根据土样中151株病原菌尖孢镰刀菌[Fusarium oxysporum f.sp.lini(FOL)]对峙培养获得21株拮抗芽孢杆菌,其中XJ2-20拮抗效果最好,盆栽实验的胡麻枯萎病防效可达56.3%,经鉴定为枯草芽孢杆菌。菌株XJ2-20对151株镰刀菌株的抑菌率最高可达64.5%,其发酵滤液可抑制尖孢镰刀菌生长及抑制分生孢子萌发。菌株XJ2-20的抑菌活性物质的最佳硫酸铵沉淀浓度为70%,活性物质对热和胰蛋白酶敏感,最适pH为7。其最佳发酵条件为LB液体培养基(初始pH 7.2)、每300mL三角瓶装液量50~100mL,29℃培养5d。
Biological control microorganism was isolated from pathogenic soil of linseed.Inhibition rates to Fusarium oxysporum f.sp.lini were evaluated using 151 fungus isolates from different provinces.A bacterial XJ2-20 from 21 antagonistic Bacillus strains showed strong antagonistic effect on the 151 fungus isolates.Its highest inhibition rate was 64.5%.Fermentation filtrate of XJ2-20 affected mycelium growth and spore bourgeoning.Its efficiency of control was as high as 56.3% in pot experiment.Optimized fermentation of XJ2-20 was under 29℃ and pH7.2 with 50 to 100mL LB medium in 300mL-flask for 5d.Antagonistic substance of XJ2-20 was extracted by 70%(NH4)2SO4 precipitation at pH7.The extracted crude protein inhibited pathogens by leading to mycelium rupture,distortion,and inhibiting bourgeoning of spores,although it was sensitive to heat and tryroteinase.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2011年第6期598-602,共5页
Chinese Journal of Oil Crop Sciences
基金
国家胡麻产业技术体系项目(CARS-17-GW-6)
关键词
胡麻
枯萎病
生防芽孢杆菌
防治效果
发酵滤液
Linseed
Fusarium wilt
Bacillus
Biocontrol effect
Fermentation filtrate