内标校准液相色谱荧光法测定食用油中苯并(a)芘

Determination of benzo[a]pyrene in edible oils by liquid chromatographyfluorescence detection with benzo[b]chrysene as internal standard

采用内标苯并(b)屈校正,建立了正相固相净化-反相液相色谱-荧光分光光度法测定食用油中苯并(a)芘的方法。正相Plus Silica固相萃取柱作为净化柱,反相C18柱作为分离柱。以苯并(b)屈作为内标,测定了苯并(a)芘的校正因子。使用本方法,苯并(a)芘在液态食用油和固态食用油中的检出限分别为0.01和0.03μg/kg。苯并(a)芘在不同食用油回收率在83%～108%之间,方法的日内精密度和日间精密度分别小于7.7%和13.2%。

The normal-phase solid phase cleaning-reversed high performance liquid chromatography-fluorescence detection was applied for the determination of the benzo[a]pyrene（BaP） in edible oils.The edible oil samples were cleansed with a Plus Silica solid phase extraction cartridge.The separation was carried out on a C18 column with gradient elution.With benzo[b]chrysene（BbC） as the internal standard,the relative correction factor（RCF） of BaP was determined by HPLC-FLD;the BaP was determined by using BbC as the single marker.The limits of detection were 0.01μg/kg and 0.03μg/kg for BaP in edible oils and in edible fats,respectively.Recoveries of BaP in different spiked edible oils were in the range from 83% to 108%.The intra-day precision and inter-day precision were less than 7.7% and 13.2% respectively.