Ki-67和E-cadherin在乳腺癌组织中的表达及意义

Expression and Its Significance of Ki-67 and E-cadherin in Breast Cancer Tissues

目的探讨增殖细胞核抗原(Ki-67)和上皮型钙黏附分子(E-cadherin)在乳腺癌组织中的表达及与乳腺癌临床病理特征的关系。方法应用免疫组化SP法检测84例乳腺癌组织中Ki-67和E-cadherin的表达,并收集肿瘤边缘5cm以上的癌旁正常乳腺组织50例为对照组。结果乳腺癌组织中Ki-67表达阳性率为88.09%,阳性蛋白定位于细胞核;E-cadherin阳性表达率为78.57%,阳性蛋白定位于细胞膜或浆;与癌旁组织相比Ki-67和E-cadherin阳性表达率差异均有统计学意义(χ2分别为65.326、5.646,P均<0.05)。Ki-67阳性表达率在不同病理类型之间差异有统计学意义(P<0.05),Ki-67的阳性表达与淋巴结转移呈正相关(r=0.241,P<0.05),与体重指数呈正相关(r=0.244,P<0.05),而与年龄、民族、月经状况、肿瘤大小、分化程度、分期之间差异无统计学意义(P>0.05),E-cadherin阳性表达与病理分级呈负相关(r=-0.239,P<0.05),在不同年龄、民族、月经状况、体重指数,以及不同肿瘤大小、分期、病理类型、是否淋巴结转移之间差异均无统计学意义(P>0.05)。E-cadherin阳性表达与Ki-67的表达强度无关。结论 Ki-67和E-cadherin与乳腺癌浸润转移有关,可作为评估乳腺癌预后、指导治疗的指标,但两者在乳腺癌发生、发展、侵袭和转移过程中没有相关性。

Objective To investigate the expressions of Ki-67 and E-cadherin in breast cancer and to analyze the relationship with clinical and pathological features of breast cancer.Methods The expression of Ki-67 and E-cadherin in 84 cases of breast cancer were detected by immunohistochemistry SP method.Results The positive expression rate of Ki-67 in patients with breast cancer was 88.09% and positive protein localized in the nuclei.The positive expression rate of E-cadherin in patients with breast cancer was 78.57% and positive protein localized in the cellmembrane or cytoplasm.The positive expression rate of Ki-67 showed significant difference among different pathological types（P〈0.05）.The expression of Ki-67 showed significantly positive correlation with axillary lymph node status（r=0.241,P〈0.05）and BMI（r=0.244,P〈0.05）.The expression of Ki-67 showed no correlation with age,ethnicity,menopausal status,tumor size,histological grade and clinical stage（P〉0.05）.The expression of E-cadherin had negative correlation with histological grade（r=-0.239,P〈0.05）.The expression of Ki-67 had no correlation with age,ethnicity,menopausal status,tumor size,BMI,clinical stage,axillary lymph node status（P〉0.05）.The expression of Ki-67 was not related with the expression of E-cadherin.Conclusion The Ki-67 and E-cadherin are important factors in the development and invasion of breast cancer metastasis.They will be used to evaluate breast cancer prognosis and to guide treatment.But the two indices do not have synergy in development,invasion,and transfer process of breast cancer.