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凡纳滨对虾BTF3基因的克隆及表达分析 被引量：2

Molecular Cloning and Expression Analysis of BTF3 in Litopenaeus vannamei
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摘要 通过电子克隆所得序列设计引物,采用RT-PCR方法首次克隆得到长655 bp的凡纳滨对虾通用转录因子3(Basic Tran-scription Factor 3,BTF3)基因序列,其中包括597 bp的完整开放阅读框,共编码198个氨基酸残基,推算分子量约45.79 kDa,理论等电点为4.93。氨基酸序列分析表明,BTF3基因编码的氨基酸序列中具有保守的NAC结构功能域,利用实时荧光定量PCR检测凡纳滨对虾不同组织的表达情况发现,该基因在凡纳滨对虾组织中广泛表达,其中肌肉组织表达量最高,在肠中表达量最低。 A pair of primers were designed according to the sequence from electronic cloning,and a 655 bp cDNA of BTF3 in Litopenaeus vannamei was first obtained by RT-PCR.The cDNA contained a 597 bp open reading frame（ORF） encoding a 198 amino acids peptide.The molecular weight of BTF3 was 45.79 kDa,and its calculated isoelectric point was 4.93.Sequence alignment revealed that the amino acid sequence of BTF3 from different species were highly conserved and contained a NAC domain.Real-time PCR results showed that BTF3 mRNA could be detected in most of Litopenaeus vannamei tissues,and the highest expression level was observed in muscle,the lowest in intestine.
作者 殷勤 彭金霞 崔亮 谢达祥 王志伟 李奎 陈晓汉
机构地区 广西壮族自治区水产研究所 中国农业科学院北京畜牧兽医研究所
出处 《西南农业学报》 CSCD 北大核心 2011年第6期2396-2400,共5页 Southwest China Journal of Agricultural Sciences
基金 广西壮族自治区直属公益性科研院所基本科研业务费(2060302GXIF-2010-04) 现代农业产业技术体系建设专项资金(编号:CARS-47) 广西科学研究与技术开发计划(桂科攻10100007-2) 国家科技支撑计划项目(2008BADB9B03)
关键词 凡纳滨对虾 BTF3 基因克隆 表达分析 Litopenaeus vannamei BTF3 Gene cloning Expression analysis
分类号 S945.1 [农业科学—水产养殖]
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  • 2郭坤,刘银坤.小热休克蛋白家族的研究现状[J].国际检验医学杂志,2006,27(4):318-320. 被引量：5
  • 3韦雪芳,王冬梅,刘思,周鹏.信号肽及其在蛋白质表达中的应用[J].生物技术通报,2006,22(6):38-42. 被引量：64
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  • 6Chen Z, Cheng C H,Zhang J F, Cao L X, Chen L,Zhou L H, Jin Y D,Ye H,Deng C,Dai Z H,Xu Q H,Hu P,Sun S H, Shen Y, Chen L B. 2008. Transcriptomic and genomic evolution under constant cold in Antarctic notothenioid fish[J]. Proceedings of the National Academy of Sciences of the United States of America, 105(35) : 12944-12949.
  • 7Colinet H,Lee S F,Hoffmann A. 2010. Temporal expression of heat shock genes during cold stress and recovery from chill coma in adult Drosophila melanogaster[J]. FEBS Journal, 277( 1 ) : 174-185.
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  • 9Huang L H, Wang C Z, Kang L. 2009. Cloning and expression of five heat shock protein genes in relation to cold hardening and development in the leafminer, Liriomyza sativa [J]. Journal of Insect Physiology, 55(3): 279-285.
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西南农业学报
2011年 第6期

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