降脂饮对胰岛素抵抗大鼠骨骼肌转运蛋白-4mRNA的影响

Influence of JZY on the Expression of GLUT-4 mRNA in Insulin Resistance Rats

目的:观察降脂饮对胰岛素抵抗大鼠骨骼肌转运蛋白-4mRNA的影响,探讨降脂饮改善胰岛素抵抗及代谢综合征的作用机制。方法:1.胰岛素抵抗大鼠模型采用高脂膳食喂养配合小剂量注射链脲佐菌素的方法复制,以胰岛素敏感指数检测模型是否被成功建立;2.除正常组外,将成模大鼠随机分为4组:模型组、降脂饮高剂量组、降脂饮低剂量组、罗格列酮组,分别连续灌胃给药6周;3.检测各组大鼠胰岛素敏感指数,并以RT-PCR法检测各组大鼠骨骼肌GLUT-4 mRNA的表达。结果:与空白组比较,模型组大鼠胰岛素敏感指数下降,差异极显著(P<0.01),说明胰岛素抵抗大鼠模型被成功建立。与模型组比较,降脂饮组、罗格列酮组大鼠胰岛素敏感指数明显上升(P<0.01);大鼠骨骼肌中GLUT-4 mRNA的表达相对增强,其中降脂饮高、低剂量组(P<0.01),罗格列酮组(P<0.05)。结论:降脂饮可明显提高胰岛素抵抗大鼠的胰岛素敏感指数,且可促进骨骼肌中GLUT-4 mRNA的表达。

Objective：To observe the influence of JZY on the expression of GLUT-4 mRNA in resistance and metabolism syndrome.Method：1.The IR model was set up in rats by feeding them high fat diet combining small dose of STZ injecting.2.Different doses of JZY were used to treat the IR rats compared with rosiglitazone.3.Differences within each group in insulin sensitivity and the expression of mRNA of GLUT-4 were observed.Result： Compared with blank group,the insulin sensitive index of model group rats had a remarkable fall（P0.01）;Compared with model group,the insulin sensitive index of JZY group and rosiglitazone group rats ascended significantly（P0.01）,and the expression of mRNA of GLUT-4 had a remarkable enhancement in JZY group（P0.01） and in rosiglitazone group（P0.05）.Conclusion：JZY can remarkably enhance insulin sensitive index and the expression of mRNA of GLUT-4 in IR rats.