苹果遗传连锁图谱构建及抗早期落叶病的基因定位

Genetic Mapping and Localization of the Early Deciduous Disease Resistant Gene in Apple

以"秦冠"×"富士"F1群体的158个株系为试材,得到最佳SSR-PCR反应体系:在20μL总的反应体系中Taq DNA聚合酶0.5U、引物0.1μmol/L、Mg2+1.8mmol/L、模板DNA浓度15ng/μL、dNTPs 0.30mmol/L。利用SSR分子标记,采用JoinMap 3.0软件构建"秦冠"×"富士"的遗传连锁图谱。结果表明:从340对SSR引物中筛选出49对具有多态性的引物,在群体中共获得75个SSR标记,其中17个不符合孟德尔遗传规律,其余27个位点可定位到10个连锁群上。对苹果杂交F1代早期落叶病进行抗性遗传分析,最后将抗早期落叶病基因定位到了遗传连锁图谱中的第10个连锁群上。与已经发表的苹果遗传连锁框架图对比结果表明,抗早期落叶病基因被定位在已发表图谱的第8连锁群上。

With the material of apple cultivar ＇Qinguan＇ as the maternal parent,＇Fuji＇ as male parent and their F1 158 progenies,an optimized PCR reaction system for SSR were obtained：in a 20 μL total volume,Taq DNA polymerase 0.5 U,primer 0.1 μmol /L,dNTPs 0.3 mmol/L,template DNA 15 ng/μL and Mg2＋ 1.8 mmol/L.A consensus genetic map from ＇Qinguan＇× ＇Fuji＇ was constructed by JoinMap 3.0 with SSR markers.The results showed that 340 SSR primer pairs,49 were proved having polymorphism between father and mother parents,and 75 SSR markers were acquired by the polymorphic primer pairs.Among these SSR markers,17 were distorted from the Mendelin ratio,27 were located on the genetic linkage map composed by 10 linkage groups and early deciduous disease resistance gene location to the tenth linkage groups,compared with the already published apple genetic linkage：early deciduous disease resistant gene was positioned in published maps of the eighth linkage groups.