一品红的组织培养研究

Study on Tissue Culture of Euphorbia pulcherrima

以一品红腋芽、顶芽、茎段、嫩叶为初次诱导外植体,对一品红的组织培养途径进行研究,建立了一品红植株再生体系。结果表明:组织培养的取材部位为腋芽、顶芽和茎段。诱导愈伤组织外植体为茎段,培养基为MS+6-BA 0.5mg/L+2,4-D 2.0mg/L+蔗糖30g/L。诱导丛生芽的外植体为腋芽、顶芽和愈伤组织,培养基配方为腋芽、顶芽:MS+6-BA 0.6mg/L+NAA0.1mg/L+蔗糖30g/L;愈伤组织:MS+6-BA 2.0mg/L+NAA 0.1mg/L+蔗糖30g/L。丛生芽继代增殖的培养基配方为MS+6-BA 0.5mg/L+NAA 0.1mg/L+蔗糖30g/L。生根培养基配方1/2MS+NAA 1.0mg/L+蔗糖30g/L。

Axillary buds,apical buds,stems and leaves of Euphorbia pulcherrima were used as explant,tissue culture of Euphorbia pulcherrima way and establishment system of plant regeneration were studied.The results showed that the best explants were axillary buds,buds and stems.The best explants of callus was stems,the most favorable media was MS＋6-BA 0.5 mg/L＋2,4-D 2.0 mg/L＋sucrose 30 g/L.The best explants of induced buds was axillary buds,apical buds and callus,the most favorable media of apical bud and axillary bud as explant：MS＋6-BA 0.6 mg/L＋NAA 0.1 mg/L＋sucrose 30 g/L;The most favorable media of callus as explant：MS＋6-BA 2.0 mg/L＋NAA 0.1 mg/L＋sucrose 30 g/L.The subculture media was MS＋6-BA 0.5 mg/L＋NAA 0.1 mg/L＋sucrose 30 g/L.The rooting media 1/2MS＋NAA 1.0 mg/L＋sucrose 30 g/L.