平欧杂种榛微繁技术研究

Study on Micropropagation of Hybrid Hazelnut

以杂种榛的4个优良抗寒栽培品种"薄壳红"、"玉坠"、"平顶黄"、"达维"及品系等春梢及夏梢幼嫩茎段作为外植体,筛选杂种榛组织培养最佳外植体、各个培养阶段的最佳培养基及最佳移栽基质,建立一个较为完整的平欧杂种榛组织培养繁育技术体系。结果表明:外植体采用6月取夏梢幼嫩茎段比较适宜,茎段分化培养采用培养基:DKW(改良)+BA 4.0+IBA 0.01+GA30.2+VH 0.1+VB510+LH 300,葡萄糖30g/L,琼脂5g/L;"薄壳红"、"玉坠"最佳增殖培养基为:DKW(改良)+BA 5.0+IAA 0.01+GA30.2+VH 0.1+VB510+LH 300,葡萄糖30g/L,琼脂5g/L,增殖系数达到3.0～3.5;杂种榛最佳生根培养基为:MS+IAA 0.5mg/L+IBA 0.8mg/L,白糖20g/L,琼脂5g/L,平均生根率83.2%;依照试验确定蛭石、草炭(1∶1)是最佳移栽驯化基质,移栽成活率达到90.5%。

＇Bokehong＇,＇Yuzhui＇,＇Pingdinghuang＇,＇Dawei＇four excellent cultivar varieties of hybrid hazelnut and other lines etc.young summer shoot and spring shoot were chosen as the explants to establish a more complete technological system of micropropagation,the optimal culture medium and conditions were selected,optimum explants and the appropriate medium for respective stages and the excellent transplanting matrix was obtained.The results showed that June was the best sampling period for collecting materials,and the appropriate media for initiation were：DKW（improve）＋BA 4.0＋IBA 0.01＋GA30.2＋VH 0.1＋VB510＋LH 300;for differentiation and multiplication were：DKW（improve）＋BA 5.0＋IAA 0.01＋GA30.2＋VH 0.1＋VB510＋LH 300,the multiple was up to 3.0~3.5;the effective medium for rooting was MS＋IAA 0.5 mg/L＋IBA 0.8 mg/L;the mean rate of rooting was 83.2%;with the experiment,vermiculite and peat（1∶1） was the excellent matrix,transplanting surviving rate was 90.5%.