外周血CK-19评价乳腺癌术后化疗及监控血液微转移

Evaluation of chemotherapy after surgery and monitoring of breast cancer micrometastases in the peripheral blood with CK-19

目的以外周血CK-19为指标评价术后化疗作用及其监控血液微转移意义。方法以120例乳腺癌术后患者为研究对象,巢式RT-PCR检测外周血CK-19 mRNA,比较化疗前后表达差异及CK-19表达与乳腺癌远处转移关系。结果化疗前血液微转移阳性率为23.33%,化疗后为3.33%,差异有统计学意义(P<0.01);CK-19阳性组肿瘤转移率为76.9%,阴性组肿瘤转移率为8.6%,两组差异有统计学意义(P<0.01)。结论术后辅助化疗能降低乳腺癌血液微转移;外周血CK-19能监控乳腺癌血液微转移。

Objective With CK-19 in the peripheral blood as indicator to assess the role of postoperative chemotherapy and monitoring of blood micrometastases. Methods 120 breast cancer patients involved in the study, nested RT-PCR was used to detect CK-19 mRNA in peripheral blood, and the expression of CK-19 before and after chemotherapy, and the relationship between CK-19 expression and distant metastasis of breast cancer were compared. Results The rate of micrometastasis was 23.33% before chemotherapy, 3.33% after chemotherapy, and the difference was signifieant（P 〈0.01 ）; the tumor metastasis rate was 76.9% in CK-19 positive group, 8.6% in CK-19 negative group, and the difference was significant （P 〈0.01 ）. Conclusion Postoperative chemotherapy can reduce breast cancer micrometastases in blood. CK-19 in peripheral blood can monitor breast cancer mierometastases.