螺旋藻培养液中碳、氮、磷的优化及其效应评价

Optimization of carbon,nitrogen and phosphorus in spirulina culture medium and its effect

以总蛋白含量和比生长速率为评价指标,进行综合评分后,优化了螺旋藻培养液中碳、氮、磷源的种类和浓度,其中,碳源为NaHCO37.00g/L、氮源为尿素0.10g/L、磷源为KH2PO40.10g/L。与对照Zarrouk配方相比,该优化配方不但可以降低螺旋藻的生产成本,而且显著提高了螺旋藻总蛋白含量、色素蛋白含量和类胡萝卜素含量。

Using the content of total protein and specific growth rate as evaluation indexes, the optimal carbon, nitrogen and phosphorus in spirulina culture medium was obtained by colligation score as follows： NaHCO3 7.00 g/L, urea 0.10 g/L, KH2PO4 0.10 g/L. Compared with Zarrouk medium, the optimal medium not only decreased its cost, but also increased the content of total protein, pigment protein and carotenoid of spirulina.