大花萱草组织培养快速繁殖

Rapid Clonal Propagation of Hemerocallis fulva L.with Tissue Culture Technique

以大花萱草幼嫩的花茎为外植体,接种在 MS+0.4mg·1^(-1)IBA+4.0mg·1^(-1)BA 的培养基上,培养1月后外植体形成了愈伤组织,再继续培养30至40天从愈伤组织上分化了不定芽。在继代培养过程中,不定芽的增殖率以在 MS+0.5mg·1^(-1)BA+0.5mg·1^(-1)KT+600mg·1^(-1)水解乳蛋白和 MS+1.0mg·1^(-1)NAA+5.0mg·1^(-1)BA 上较佳。试验结果表明,光照强度及光暗培养对大花萱草不定芽的增殖无明显影响;经一次使用的培养基可以再次利用;而且,以食用蔗糖代替培养基中的化学纯蔗糖对不定芽的增殖有促进作用。大花萱草试管新梢的生根较容易,生根率可达100%。本文还对大花萱草通过组织培养技术进行快速繁殖生产种苗以及降低成本进行了讨论。

The young flower stalks of Hemerocallis fulva were used as explants and cultured in the me- dium of MS+0.4mg·l^(-1)IBA+4.0mg·l^(-1)BA.The explants formed into callus after 1 month culture.And 30 to 40 days later,adventitious buds were differentiated from the callus.During subculture,the media of MS+0.5mg91^(-1)BA+0.5mg·l^(-1)KT+600mg·l^(-1)lactalbumin hydrolysate and MS+1.0mg·1^(-1) NAA+5.0mg·1^(-1)BA were better for the increase of adventitious buds.The study showed that light inten- sity,intermittent illumination had no significant effects on the development of adventitious buds.The medium after one culture could be reused once again for subculture;and commercial cane sugar which replaced the sucrose in the medium stimulated the adventitious bud formation in proper concentrations.The shoots of Hemerocallis fulva obtained in the experiment formed the root easily and the rate was as high as 100%.The possibility of using rapid clonal propagation via tissue culture for Hemerocallis fulva and the cost reduction of culture in vitro were also discussed.