培养基对香石竹试管苗生长及其玻璃化的影响

Effect of Media on the Rapid Clonal Propagation of Carnation in Vitro and Its Vitrification:

本文探讨了香石竹组织培养系统和玻璃化问题。结果表明,在pH值5.8～7.0的范围内,香石竹嫩梢均可正常生长,生根的适宜pH值为6.7～7.0。食用蔗糖可代替化学纯蔗糖用作香石竹培养的碳源。香石竹最适的增殖培养基为Ms+0.1mg/l BA+0.1mg/l KT+0.1mg/l NAA。培养基中添加10～15mg/l CCC或0.5～1.0mg/l PP_(333)可导致嫩梢矮化、粗壮,减少玻璃化。通过培养基的筛选,可以将玻璃化率控制在5～10％的范围内。在培养过程中存在着玻璃梢与正常梢的互相转化,茎的异质性是引起转化的最重要因素。

In this paper, the culture system and vitrification of carnation in vitro were studied.The results indicated that pH of the medium in the range of 5.8 to7.0 did not affect the growth of carnation shoots and the optimal pH for the root induction and growth of the shoots was ranging from pH 6.7 to 7.0.Commercial cane sugar could be used as carbon source of the medium instead of using sucrose (CP) .The most suitable medium for the growth and multiplication of carnation shoots was MS medium supplement with 0.1 mg/ 1 BA+0.1 mg/ 1 KT+0.1mg/ 1 NAA.It was found that adding 10-15 mg/ 1 CCC or 0.5-1.0mg/1 PP_(333) to the medium resulted in shoots dwarf and thickness and also reduced percent of vitrification which could be reduced to less than 5o by medium screening. The vitrification shoots and normal shoots transformed each other in the course of culture.The stem heterogeneity was the main cause of the transformation.