菝葜皂苷元对结肠癌细胞Lovo黏附和侵袭能力的影响

Effect of Sarsasapogenin on the Adhesion and Invasion Capabilities of Human Colon Cell Line Lovo

目的:本研究旨在探讨菝葜皂苷元体外对人结肠癌细胞Lovo增殖、黏附、侵袭的影响。方法:采用MTT法、黏附实验、Transwell小室侵袭实验,观察菝葜皂苷元对人结肠癌细胞Lovo增殖、黏附和侵袭能力的影响。结果:①菝葜皂苷元有抑制人结肠癌细胞Lovo增殖的作用,与对照组比较,随着药物浓度的增大,其对细胞的抑制率也明显增大(P<0.01)。②菝葜皂苷元作用人结肠癌细胞Lovo后,细胞的黏附能力明显降低(P<0.01)。③菝葜皂苷元作用人结肠癌细胞Lovo 24h后,侵袭的细胞数较对照组明显减少(P<0.01)。结论:菝葜皂苷元对人结肠癌细胞Lovo的增殖具有抑制作用;菝葜皂苷元能抑制人结肠癌细胞Lovo的黏附能力和侵袭能力。

Objective ：This study was designed to explore ：The effect of sarsasapogenin on the biological behaviors including proliferation, adhesion and invasion capabilities of human colon cell line Lovo in vitro. Method：After human colon cell line Lovo was treated with sarsasapogenin at different concentrations, cell proliferation and adhesion capabilities was assessed using MTT assay. The invasion capabilities of Lovo cells were determined using transweil method （Boyden chamber）, respectively. Results ：（1）Sarsasapogenin significantly inhibited the proliferation of Lovo ceils （P〈0.01）.（2） Sarsasapogenin significantly decreased the adhesion capability of Lovo cells （P〈0.01）.（3）Sarsasapogenin significantly decreased the invasion capability of Lovo cells （P〈0.01）. Conclusion：Sarsasapogenin inhibited the proliferation of human colon cell line Lovo.Sarsasapogenin supressed the adhesion and invasion capability of Lovo cells.