淫羊藿HPLC指纹图谱的建立与产地、部位成分差异性研究

Establishment of HPLC Fingerprint of Epimedium and Composition Studies of Origin and Site

目的:建立淫羊藿的HPLC指纹图谱,并分析不同产地、部位的品质差异性。方法:Kromasil C18柱(250mm×250mm×4.6mm,5μm),检测波长为270nm;流速为1mL/min;柱温为室温;乙腈和1%醋酸为流动相,进行梯度洗脱;测定湖北、东北、吉林不同产地茎、叶指纹图谱,比较其差异性。结果:淫羊藿药材指纹图谱共检出23个峰,峰面积之和大于总峰面积的90%,其精密度、稳定性、重复性均符合《中药注射剂指纹图谱研究的技术要求(暂行)》中的有关规定。结论:本方法可科学、合理地评判淫羊藿药材品质的差异,对于淫羊藿道地药材研究具有一定的意义。

Objective ： To establish the HPLC fingerprint of Epimedium, and analyze quality differences of different origin and location. Methods ： Kromasil C18 column （ 250mm×250mm×4.6mm, 5 μ m ）, detection wavelength 270nm ; flow rate lmL/min ; column temperature was at room temperature ; acetonitrile and 1% acetic acid as mobile phase, gradient elution ; determine fingerprints of different origin stems, leaves from Hubei, Northeast, Jilin, and compare their differences. Results ： Epimedium Fingerprint has 23 peaks, the total peak area is greater than 90% . Its precision, stability, repeatability are in line with the relevant provisions in Fingerprint Of Traditional Chinese Medicine Injection of Technical Requirements（ Temporary ）. Conclusion ： This method can scientifically and reasonably judge the quality differences of Epimedium, and has a certain significance for Epimedium genuine medicine research.