改良法建立大鼠深部浸润型子宫内膜异位症模型

Establishment of Modified Deep Infiltrating Endometriosis Model in Rats

目的采用改良法通过自体移植在大鼠子宫膀胱间建立深部浸润型子宫内膜异位症(DIE)模型并探讨其优缺点。方法移植大鼠自体子宫内膜于子宫膀胱间腹膜处,同时切除宫旁游离脂肪建立改良子宫膀胱间DIE模型,并与常规直肠子宫陷凹处DIE模型及非改良子宫膀胱间DIE模型作对比。术后2周再次手术观察病灶生长情况,并对病灶粘连程度进行评分;同时对改良子宫膀胱间DIE模型病灶进行组织形态学、组织化学观察。结果 3组均成功建立DIE模型,体积大小差异无统计学意义。直肠子宫陷凹处DIE模型和非改良子宫膀胱间DIE模型的病灶粘连评分分别为2.38±0.74和2.75±0.46,改良子宫膀胱间DIE模型病灶粘连评分为1.00±0.50,较前两组明显减小(P<0.05),且具有与在位子宫内膜相似的形态和结构。结论采用改良法建立子宫膀胱间DIE模型操作简单、病灶粘连轻,适用于实验研究。

Objective To explore the establishment of rat models of modified deep infiltrating endometriosis（DIE） in the peritoneum between the uterus and bladder by autologous transplantation and discuss the advantage and disadvantage of the models.Methods Modified DIE models were established by transplanting endometrium to peritoneum at the junction between the uterus and bladder in rats,at the same time we removed parametrial free fat during the operation.Moreover,we contrasted them with DIE models at Douglas＇ cul-de-sac and non-improved DIE models in the peritoneum between the uterus and bladder.After two weeks,the rats were given the second operation.The adhesion degree of the implants was quantitatively evaluated.At the same time we observed histomorphology and histochemistry of modified DIE lesions.Results Rat DIE models were established successfully in all 3 groups,and the mean volume of the lesions showed no significant difference（P 0.05）.The adhesion scores in the DIE models at Douglas＇ cul-de-sac and non-modified DIE models at the junction between the uterus and bladder were 2.38±0.74 and 2.75±0.46 respectively.The adhesion score in the modified DIE models at the junction between the uterus and bladder was 1.00±0.50,which reduced significantly compared with the other two groups（P 0.05）.It showed that endometrial structure of lesions was similar to eutopic endometrium.Conclusion DIE models at the junction between the uterus and bladder in rats established by modified method are suitable for study due to simple manipulation and less adhesion.