猫爪草中吡咯丁酸的含量测定研究

Study on Quality Control of Ranunculi Ternati Radix

目的探寻猫爪草中指标性成分,并建立HPLC测定猫爪草中指标性成分含量的方法,对猫爪草进行质量控制研究。方法采用不同柱色谱技术进行分离,通过光谱、波谱分析鉴定指标性成分吡咯丁酸的化学结构。采用高效液相色谱法进行吡咯丁酸的含量测定,以Agilent TC-C18柱(4.6 mm×250 mm,5μm)色谱柱为固定相;流动相为0.4%醋酸水-甲醇;检测波长:292 nm;体积流量:1.0 mL.min-1。结果猫爪草中指标性成分经分离、鉴定为吡咯丁酸,吡咯丁酸在0.372～1.302μg线性关系良好,r2=0.999 8。平均回收率为100.89%(RSD=0.83%)。结论本方法简单、快速、可靠,为猫爪草的质量控制提供了科学依据。

ABSTRACT ： OBJECTIVE To identify a characteristic chemical marker of Ranunculi Ternati Radix and establish a HPLC method for determination of its content. METHODS Various chromatographic methods were applied to isolate the marker 4,[ formyl-5- （hydroxymethyl）-lH-pyrrol-l-yl] butanoic acid, and its structure was elucidated by spectroscopic methods, The HPLC was performed on Agilent TC-C18 column（4. 6 mm × 250 mm, 5 μm） Using CH30H-H2O （ containing 0. 4% acetic acid）as the mobile phase, The flow rate was 1.0 mL ·min ^- 1 and the detection wavelength was set at 292 nm. RESULTS 4- [ formyl-5- （hydroxymethyl） -1H-pyrrol- 1-yl] butanoic acid was isolated and identified as the chemical marker. The HPLC method showed good lineafity within：the range of 0. 372 - 1. 302 μg （ r2 = 0. 999 8 ）. The average recovery of 4- [ formyl-5- （hydroxymethyl） -1H-pyrrol-l-yl ] butanoic acid was 1001 89% （ n =6,RSD =0. 83% ）. CONCLUSION This method is simple, rapid and reliable for the quality Control of Ranunculi Ternati Radix.