活血助孕方对输卵管炎性不孕大鼠sICAM-1表达的研究

Research on Activating Blood and Promoting Pregnancy Formula for the sICAM-1 Expression of Infertile Rats with Salpingitis

目的:探讨中药活血助孕方对慢性炎性不孕大鼠可溶性细胞粘附因子(sICAM-1)的影响及其疗效机理,并比较高剂量组、中剂量组、低剂量组三种剂量的疗效。方法:以Wista雌性大鼠为受试对象,用混合菌接种法复制输卵管炎性阻塞性不孕模型,80只大鼠随机分为正常对照组、模型组、活血助孕方低剂量组、中剂量组、高剂量组,治疗后用免疫组化法检测sICAM-1的表达。结果:活血助孕方高、中、低剂量组能不同程度恢复模型大鼠抑制sICAM-1表达。活血助孕方高剂量组有效率明显高于活血助孕方中剂量组和低剂量组。结论:用混合菌接种法可以复制大鼠输卵管炎性阻塞性不孕模型;活血助孕方能改善输卵管炎性大鼠输卵管结构及功能,活血助孕方能够抑制sICAM-1表达,从而发挥其抗炎症作用。

Objective：To study the impacts and curative effect mechanism of activating blood and promoting pregnancy formula on the chronic inflammatory infertile rats′ cell factor sICAM-1,and to compare the curative effect of high-dose group,middle-dose group and low-dose group respectively.Methods：Wista female rats were taken as study subjects,and mixed bacterial inoculation method was used to duplicate the obstructive infertility model with salpingitis.80 rats were randomly divided into normal control group,model group,low-dose group,middle-dose group and high-dose group treated with activating blood and promoting pregnancy formula.After treatment,immunohistochemical method was used to detect sICAM-1 expression.Results：Activating blood and promoting pregnancy formula in low-dose group,middle-dose group and high-dose group could regain model rats′ ability of restraining sICAM-1 expression in varying degrees.The effective rate of activating blood and promoting pregnancy formula in high-dose group was obviously superior to the low-dose group and middle-dose group.Conclusion：Mixed bacterial inoculation method can be used to duplicate the obstructive infertility model with salpingitis;Activating blood and promoting pregnancy formula can improve the construction and function of oviduct in rats with salpingitis;Activating blood and promoting pregnancy formula can restrain sICAM-1 expression to exert its role in anti-inflammation.