核移位YB-1蛋白、P-gp及PCNA在肝癌中的表达及意义

The expression and significance of YB-1 with nuclear translocation,P-gp and PCNA in hepatocellular carcinoma

目的探讨核移位YB-1蛋白、P糖蛋白(P-gp)、增殖核抗原(PCNA)在肝细胞肝癌(hepatocellular carcinoma,HCC)组织中的表达及其在肝癌发生发展中的作用。方法采用免疫组化法检测YB-1、P-gp、PCNA蛋白在58例HCC组织、20例正常肝组织中的表达。结果在肝癌组织中核移位YB-1与P-gp、PCNA蛋白阳性率分别为31%(18/58)、83%(48/58)、100%(58/58),与正常肝组织相比有显著差异(P<0.05)。核移位YB-1蛋白及PCNA积分与肝癌病理分级、门静脉癌栓、肿瘤大小有关(P<0.05);P-gp蛋白与肝癌病理分级有关(P<0.05)。核移位YB-1蛋白与P-gp、PCNA蛋白表达呈正相关(P<0.05)。结论 YB-1蛋白可能通过核移位途径上调P-gp、PCNA蛋白表达,从而促进肝癌的发生发展及其恶性表型的维持,YB-1蛋白有可能成为肝癌治疗的新靶点。

Objective To observe expression levels of YB-1 protein with nuclear translocation, P-gp, PCNA protein in hepatocellular carcinoma （HCC） tissues, and explore their significance and correlation. Methods Immunochemistrywas performed in 58 HCC tissues and 20 normal liver tissues to study the levels of YB-1 protein with nuclear transloca- tion, P-gp and PCNA protein. Results Positive rates of YB-1 with nuclear translocation, P-gp, PCNA protein ap- peared at high levels in immunochemistry of HCC 3 1% （18/58） , 83% （48/58） , 100% （58/58） compared with normal liver tissues （P 〈 0.05）. Furthermore, the nuclear expression of YB-1 and the PCNA were significantly correlated with the pathological grade, tumor size and portal venous invasion （P 〈 0.05 ）. P-gp was correlated with the pathologicalgradeonly （P 〈 0.05）. The protein of YB-1 with nuclear translocation was significantly correlated with P-gp and PCNA protein （P 〈 0.05）. Conclusion The data demonstrate that YB-1 protein with nuclear translocation may up regulate the P-gp, PCNA protein which may be involved in human HCC carcinogenesis and malignant phenotype, and YB-1 may be a special treatment target for HCC.