新型抗钙化牛颈静脉管道材料的体外细胞毒性评价

In vitro cytotoxicity evaluation of a new type anticalcification bovine jugular venous conduit

背景:TritonX-100、环氧氯丙烷联合改性处理戊二醛固定的牛颈静脉管道是一种新型抗钙化右心管道材料,其生物相容性方面的研究较少。目的:评价新型抗钙化牛颈静脉管道的体外细胞毒性。方法:通过CCK-8法检测新型抗钙化牛颈静脉管道(实验组)及单纯戊二醛处理牛颈静脉管道材料浸提液(对照组)对L-929小鼠成纤维细胞的毒性作用,以第2,4天为检测时间点,计算细胞相对增殖率、对材料毒性进行分级。结果与结论:CCK-8法细胞毒性试验显示新型抗钙化牛颈静脉管道材料浸提液第2,4天L-929细胞增殖率均在85%以上,毒性分级为1级,无细胞毒性,且显著优于对照组(P<0.05)。提示经戊二醛、TritonX-100、环氧氯丙烷联合处理制备的新型抗钙化牛颈静脉管道材料无细胞毒性。

BACKGROUND： Triton X-100 and epoxy chloropropane modification with glutaraldehyde-treated bovine jugular venous conduit is a new type anticalcification valved conduit for right ventricular outflow tract reconstruction. There are few reports on the biocompatibility research. OBJECTIVE： To evaluate the cytotoxicity of the new type anticalcification bovine jugular venous conduit in vitro. METHODS： The cytotoxicity of the new type anticalcification bovine jugular venous conduit （experimental group） and only glutaraldehyde treated bovine jugular venous conduit extracts （control group） was detected by CCK-8. The relative growth rate was calculated and the toxicity was graded at days 2 and 4. RESULTS AND CONCLUSION： CCK-8 test results showed that the relative growth rate of the experimental group was more than 85%. The toxicity grade was 1, and there was non-cytotoxicity, which were significantly better than those in the control group （P 0.05）. It was indicated that Triton X-100 and epoxy chloropropane modification with glutaraldehyde-treated bovine jugular venous conduit had no cytotoxicity and should be a safe implanted material.