胶原喷剂修复兔鼻黏膜的机械损伤

Repair of mechanical damage of rabbit nasal mucosa by collagen spray

背景:胶原是一种天然的低免疫原性物质,具有良好的细胞相容性和组织相容性,能促进血管生成,在细胞分化、组织修复、器官的正常营养等方面都有着极其重要的作用。目的:评价胶原喷剂对新西兰兔鼻黏膜机械损伤的修复作用。方法:制备鼻黏膜机械损伤模型,给予治疗组左鼻胶原喷剂,模型组左鼻同量生理盐水,观察创伤部位的修复情况及鼻黏膜的病理变化。结果与结论:胶原喷剂无刺激性。治疗组杯状细胞计数、黏膜下腺体与模型组比较有降低趋势,但差异无显著性意义。治疗组黏膜下水肿情况与模型组比较明显减轻,差异有显著性意义(P<0.01)。组织形态学观察结果发现,新生修复的上皮杯状细胞数量减少,黏膜下腺体为小圆形,形态规则,分泌通畅,无明显水肿,且腺体密集;模型组则无上述改变。提示胶原喷剂对黏膜损伤具有明显的修复作用,黏膜固有层炎细胞浸润现象减轻,无明显弥漫性黏膜下水肿表现,腺体分泌情况良好。

BACKGROUND： Collagen is a kind of natural material, which has a low immune, good cell compatibility and tissue compatibility. It can promote the generation of vascular and plays an extremely important role in cell differentiation, tissue repair and the normal nutrition to organs. OBJECTIVE： To evaluate the repair function of collagen spray on mechanical damaged nasal mucosa in rabbits. METHODS： Nasal mucosa mechanical damage model was prepared. Collagen sprays were given to the treatment group in the left nose, and the left nose of model group was treated with the same amount of saline. The repair situation and pathomorphology of the wounded tissue were observed. RESULTS AND CONCLUSION： Collagen spray had no excitant. The goblet cell count and submucosal glands in the treatment group were slightly decreased than the model group. The rate of submucosal edema in the treatment group was significantly different from that in the model group （P 0.01）. Under light microscope, there was a decrease of fresh repaired epithelial goblet cells, the submucosal glands were small and well-regulated, and its secretion was unobstructed; there were no obvious edema, and the glands were dense; none of the above could be seen in the control group. The collagen spray had a clear repair function on mucosal injury, the infiltrating phenomenon of inflammatory cells was reduced in the mucosal lamina propria, the performance of diffuse submucosal edema was not obvious, and the glandular secretion was in good condition.