人骨髓间充质干细胞检测表面氟化处理镁合金材料的细胞相容性

Cytocompatibility of magnesium alloys after surface fluoridation tested by human bone marrow mesenchymal cells

背景:表面氟化处理的AZ31B镁合金是中科院金属研究所新研制的镁合金,是否具有良好的生物相容性尚不确切。目的:以人骨髓间充质干细胞作为检测细胞,评价表面氟处理的镁合金材料的细胞相容性。方法:人骨髓间充质干细胞培养并予以鉴定,将镁合金AZ31B作为对照组,氟处理的镁合金AZ31B材料为实验组。以人骨髓间充质干细胞作为检测细胞,取两组材料浸提液进行体外细胞相容性实验,评价氟处理的镁合金AZ31B材料的生物相容性。结果与结论:与未经氟处理镁合金AZ31B材料相比,经氟处理镁合金AZ31B材料能显著提高细胞存活率,细胞毒性分级1级,对细胞生长基本无毒性作用。结果表明,经氟处理镁合金AZ31B材料生物相容性优于未经氟处理镁合金AZ31B材料。

BACKGROUND：AZ31B magnesium alloy with surface flouridization is a new type of magnesium alloy developed by Institute of Metal Research,Chinese Academy of Sciences.Its biocompatibility is unclear. OBJECTIVE：To observe the cytocompatibility of magnesium alloys with surface fluoridation tested by human bone marrow mesenchymal cells（BMSCs）. METHODS：Human BMSCs were identified and cultured.The AZ31B magnesium alloy was taken as control and the AZ31B magnesium alloy with surface fluoridation was experimental group.Human BMSCs were the test cells.Leaching liquor of the alloy in both groups was used for cell compatibility experiments in vitro in order to evaluate the biocompatibility of AZ31B magnesium alloy.RESULTS AND CONCLUSION：The cell survival rate of AZ31B magnesium alloys with surface fluoridation was significantly higher than AZ31B magnesium alloys without surface fluoridation.The toxicity of cells in the leaching liquor of AZ31B magnesium alloys with surface fluoridation was Grade 1 and was non-toxic to the basic cell growth.The biocompatibility of the AZ31B magnesium alloys with surface fluoridation is superior to the AZ31B magnesium alloys without surface fluoridation.