电纺纳米羟基磷灰石/聚酯酰胺复合支架材料的制备及其细胞相容性

Preparation of nano-hydroxyapatite/aliphatic polyester-amide electrospun ultrafine fiber and its biocompatibility

背景:采用静电纺丝技术将功能性无机纳米微粒复合高分子超细纤维,形成类细胞外基质结构和功能的复合支架材料是骨组织工程支架领域一个新的研究方向。目的:通过静电纺丝法构建纳米羟基磷灰石/脂肪族聚酯酰胺复合纤维支架材料,并初步考察其细胞相容性。方法:以静电纺丝法制备纳米羟基磷灰石/脂肪族聚酯酰胺超细纤维支架材料,通过扫描电镜、原子能谱等表面形貌的物相分析,进行细胞在复合材料上的形态学观察。结果与结论:通过静电纺丝法成功制备出纳米羟基磷灰石/脂肪族聚酯酰胺超细纤维复合材料,成骨细胞直接培养于材料上呈现良好生长行为,初步证实了复合支架材料的细胞相容性。说明静电纺丝技术在构建类骨细胞外基质结构和功能的仿生复合材料方面具有独特优势,电纺超细纤维复合材料有望成为新型的骨组织工程支架。

BACKGROUND：Bioactive ceramics can be introduced into ultrafine fibers by electrospinning method.The electrospun composite which simulates the structure and biological function of native extracellular matrix is a new branch in bone tissue engineering research. OBJECTIVE：To prepare the nano-hydroxyapatite/aliphatic polyester-amide electrospun ultrafine fibers（nHA/PEA） and to evaluate the biocompatibility.METHODS：The nHA/PEA scaffold was prepared by electrospinning technology and characterized with X-ray diffraction,scanning electron microscope and energy-dispersive X-ray spectroscopy.Moreover,osteoblasts were cultured on the surface of nHA/PEA scaffold and the morphology of osteoblasts was investigated. RESULTS AND CONCLUSION：Good cell adhesion and proliferation manner was observed on the fibrous scaffold,indicating that the cellular biocompatibility of the composite scaffold is preliminarily confirmed.It is expected that the electrospun nHA/PEA composite scaffold can be a potential biomimetic extracellular matrix biomaterial for bone tissue engineering.