口服供体脾细胞对大鼠移植肾的影响

Effects of oral administration of donor spleen cells on kidney transplantation in rats

背景:口服供体抗原诱导免疫耐受已被证实有显著效果,而脾脏为人体最大淋巴器官,富含T淋巴细胞,可提供丰富抗原。目的:观察口服供体脾细胞对大鼠肾移植移植肾功能影响。方法:肾移植前脾细胞组Lewis(RT11)大鼠采取口服灌胃5×105个BN(RT1n)大鼠供体脾细胞,1次/d,持续7d;肾移植组Lewis(RT11)大鼠口服灌胃1mLPBS为对照。结果与结论:移植后第5天肾移植组出现移植肾排斥症状,脾细胞组平均存活时间长于肾移植组,移植后脾细胞组血肌酐、尿素氮水平升高明显慢于肾移植组;苏木精-伊红染色显示肾移植组移植肾发生急性排斥变化早于脾细胞组。说明口服供体脾细胞能诱导免疫耐受,延长移植肾存活时间。

BACKGROUND： It is proved that oral administration of donor antigen can induce immunology tolerance effectively. The spleen is the biggest human lymphoid organ containing many T lymphocytes and can provide abound antigens. OBJECTIVE： To observe the effects of oral administration of donor spleen cells on rat kidney function after transplantation. METHODS： Before kidney transplantation, Lewis （RT11） rats in splenocytes group were fed with 5×105 donor splenocytes by gastric intubation daily for 7 days, while Lewis （RT11） rats in transplantation group were fed with 1 mL PBS as control. RESULTS AND CONCLUSION： On the 5th day after transplantation, allografts rejection symptoms began appearing in the transplantation group. Mean survival time of splenocytes group was longer than that of the transplantation group. After transplantation, the serum creatinine and urea nitrogen level in splenocytes group increased slower than that in the transplantation group. According to hematoxylin-eosin staining, the acute rejection occurred earlier in transplantation group than that in the splenocytes group. It demonstrates that oral administration of donor spleen cells can induce immune tolerance and prolong the survival time of kidney allografts.