雷公藤多苷对同系大鼠肺移植排斥反应的影响

Effect of tripterygium glycosides on pulmonary transplantation rejection of syngeneic rats

背景:雷公藤是卫矛科木质藤本植物,其免疫抑制作用被多项研究所证实,被认为可以作为肺移植后的免疫调节剂。目的:观察雷公藤多苷对大鼠肺移植后急性排斥反应的抑制作用以及外周血细胞间黏附分子1、活化T细胞的影响。方法:随机选取50只SD大鼠分为空白组、对照组和治疗组。空白组为正常大鼠,对照组和治疗组大鼠采用改良三袖套法吻合技术建立大鼠左肺原位移植动物模型。移植后空白组和对照组用生理盐水灌胃(1mL/d),治疗组用雷公藤多苷(50mg/kg?d)灌胃,连续灌胃5d。结果与结论:治疗组移植肺排斥反应分级明显低于对照组(P<0.01);治疗组外周血CD25+T细胞数和细胞间黏附分子1含量高于空白组(P<0.05),而低于对照组(P<0.05)。结果可见雷公藤多苷可抑制大鼠肺移植后急性排斥反应,其作用机制可能与降低外周血CD25+T细胞数,拮抗肺组织细胞间黏附分子1表达有关。

BACKGROUND： Tripterygium wilfordii is woodiness lianas of celastraceae and has been proved to possess immunosuppression. Tripterygium wilfordii can be used as immunomodulator after lung transplantation. OBJECTIVE： To study the inhibition of tripterygium glycosides against acute rejection after lung transplantation of rats and its effect on the intercellular adhesion molecule 1 （ICAM-1） level and the number of activated T cells in the peripheral blood. METHODS： Fifty Sprague Dawley rats were selected randomly and divided into 3 groups： the blank group, the control group and the treatment group. Normal rats were the blank group. Lung orthotopic transplantation models were established by the modified ＂cuff-like＂ vessel anastomosis technique in the rats of the control group and the treatment group. After surgery, the blank group and the control group were irrigated the rat stomach with normal saline at 1 mL/d, while the treatment group was treated with tripterygium glycosides at 50 mg/kg/d for 5 days. RESULTS AND CONCLUSION： The rejection grade of lung transplantation of the treatment group was significantly lower than that of the control group （P 〈 0.01）; the number of CD25＋T cells and the ICAM-1 level in the peripheral blood of the treatment group were higher compared with the blank group （P 〈 0.05）, and lower than those of the control group （P 〈 0.05）. Tripterygium glycosides may reduce the peripheral CD25＋T cells and antagonize the ICAM-1 in lung tissues to inhibit the acute rejection after lung transplant in rats.