黄芪多糖对体外培养多囊卵巢综合征患者卵巢颗粒细胞雄激素分泌异常的影响

The effect of astragahis polysaccharides on androgen excess within ovarian granulosa cells of PCOS patients in vitro

目的 探索多囊卵巢综合征（PCOS）患者卵巢颗粒细胞雄激素分泌的变化及黄芪多糖对其雄激素分泌变化的影响.方法 收集行IVF/ICSI-ET 17例PCOS患者的卵巢颗粒细胞进行体外培养,用浓度为50、100、200、500、1000 μg/ml的黄芪多糖分别作用于体外培养的PCOS患者卵巢颗粒细胞,检测其培养液中雌激素（E2）、孕激素（P）、雄激素（T）水平的变化.结果 PCOS患者卵巢颗粒细胞分泌T水平[（0.4716±0.03）nmol/L]明显高于对照组[（0.23 ±0.0162） nmol/L,P＜0.05],两组E2、P水平比较差异均无统计学意义（P＞0.05）.给予不同浓度黄芪多糖处理后,PCOS组患者颗粒细胞培养液中T水平明显降低（P＜0.01）.黄芪多糖浓度分别为1000、500、200、100及50μg/ml的T水平与0μg/ml相比,其差异均有统计学意义（P＜0.01）.结论 黄芪多糖能抑制PCOS患者卵巢颗粒细胞体外培养中的睾酮分泌,降低睾酮分泌水平,可成为治疗PCOS患者的新途径.

Objective To study androgen excess within ovarian granulosa cells of PCOS patients in vitro,and the partial improvement by using astragalus polysaccharides.Methods Ovarian granulosa cells from the PCOS patients during IVF / ICSI - ET were isolated and cultured in vitro,then it was treated by different concentrations of astragalus polysaccharides.The levels of estradiol,progesterone and testosterone were determined.Results Androgen secretion within ovarian granulosa cells from PCOS patients was significantly increased [ （ 0.4716 ± 0.03 ） nmol/L,（ 0.23 ± 0.0162 ） nmol/L,P 〈 0.05 ].The levels of estradiol and progesterone were not significantly different（ P 〉 0.05 ）.Different concentrations of astragalus polysaccharides may result from the alleviation of androgen secretion which was commonly found in PCOS treatment （concentration was 1000 μg/ml,100 μg/ml,50 μg/ml,P 〈 0.01 ;concentration was 500 μg/ml,P =0.002 ; concentration was 200 μg/ml,P =0.003）.Conclusions Astragalus polysaccharides inhibit the secretion of testosterone in granulosa cells of PCOS patients during in vitro,and it may be an alternative treatment for PCOS.