耐甲氧西林金黄色葡萄球菌耐药性分析

Surveillance on Resistance of Methicillin-resistant Staphylococcus Aureus Infection

目的:了解我院耐甲氧西林金黄色葡萄球菌(MRSA)对临床常用抗菌药物的耐药情况,指导临床合理使用抗生素。方法:用PC20(美国德灵)鉴定板、头孢西丁纸片扩散法和MRSA乳胶凝集试剂盒对临床分离的69株金黄色葡萄球菌进行MRSA检测,并用PC20鉴定板做药敏实验。结果:PC20板法、头孢西丁纸片扩散法和乳胶凝集法对MRSA的检出率分别为72.5%、71.0%、72.5%,三种方法对MRSA的检出率无显著差异(p>0.05)。MRSA对万古霉素、呋喃妥因全部敏感,对复方新诺明耐药率较低,但对其他抗生素耐药率高。结论:MRSA分离率和耐药率均较高,应合理选用抗生素进行治疗并控制院内感染。

Objective：To investigate the anti-microbial resistance of methicillin-resistant staphylococcus aureus（MRSA） for the guidance of rational use of antibiotics in our hospital.Methods： PC20 identification plates,the MRSA-screen latex agglutination test,and cefoxtin disc diffusion method were performed to analyze the 69 clinical isolates of Staphylococcus aureus（SA）.The anti-microbial sensitivity tests were carried out by PC20 identification plates.Results： A total of 69 clinical SA isolates were tested.The detection rate of MRSA by PC20 identification plates,the MRSA-screen latex agglutination test and cefoxtin disc diffusion method was 72.5 %,71.0 % and 72.5 %,respectively.There was no statistically significant difference（p0.05） for the detection rate of MRSA among those methods.MRSA was susceptible to vancomycin,nitrofurantoin and trimethoprim/sulfa,and the rate of drug-resistance to other antibiotics was all very high.Conclusions： We should pay more attention to detecting MRSA and controlling nosocomial infections.The resistance of MRSA to antibiotics should be further monitored.