尾静脉移植羊膜间充质干细胞治疗大鼠脑缺血再灌注损伤

Transplantation of human amnion membrane mesenchymal stem cells via tail vein for the treatment of cerebral ischemia-reperfusion injury in rats

背景:羊膜间充质干细胞是一种理想的再生医学领域的种子细胞来源。目的:探讨尾静脉移植羊膜间充质干细胞对脑缺血损伤的作用。方法:线栓法制备大鼠大脑中动脉梗死模型,缺血2h后进行再灌注。造模后24h通过尾静脉移植1.0×105个人羊膜间充质干细胞,于造模后2,4周完成神经功能评分后取材。以未移植的模型大鼠为对照。结果与结论:脑损伤后,大鼠出现神经功能评分明显增高,羊膜间充质干细胞移植1周,大鼠的神经功能评分显著降低(P<0.05),移植2,4周时,神经功能进一步改善。免疫荧光染色显示,移植羊膜间充质干细胞的大鼠脑组织中可见较多发绿色荧光的BrdU阳性细胞,主要位于缺血区周围、皮质下和侧脑室。说明尾静脉移植羊膜间充质干细胞可迁移至大鼠脑缺血区,改善大鼠的神经功能。

BACKGROUND：Amniotic mesenchymal stem cells are an ideal seed cells sources for regenerative medicine.OBJECTIVE：To investigate the effect of human amnion membrane mesenchymal stem cells（hAMSCs） via tail vein for the treatment of cerebral ischemia injury.METHODS：The rat models of middle cerebral artery infarction were established,and underwent reperfusion at 2 hours after ischemia.1.0×105 hAMSCs were transplanted through tail vein after 24 hours cerebral ischemia and the neurological severity score（NSS） was evaluated after 2 and 4 weeks cerebral ischemia.The rat without transplantation was as control.RESULTS AND CONCLUSION：After cerebral ischemia injury,the neurological score of rats was increased significantly;at 1 week after AMSCs transplantation,the neurological score of rats was decreased significantly（P〈0.05）;at 2 and 4 weeks after AMSCs transplantation,the neurological score was further improved.Immunofluorescence staining showed that there were green fluorescence BrdU-positive cells in the rats cerebral tissue transplanted with hAMSCs,and the positive cells were mainly located around the ischemic area,subcortex and the lateral ventricle.It indicates that tail vein transplantation of hAMSCs can migrates to the ischemia area and improve the neurological function of rats.