体外诱导胎盘间充质干细胞向表皮细胞的分化

Differentiation of placenta mesenchymal stem cells to epidermal cells in vitro

背景:以往研究证明胎盘间充质干细胞经诱导可以向骨、软骨、肌肉、脂肪等间充质细胞分化。目的:探讨体外诱导胎盘间充质干细胞分化为表皮细胞的可行性。方法:分离培养胎盘间充质干细胞,流式细胞仪鉴定其表面抗原,用含体积分数为2%胎牛血清以及20μg/L表皮生长因条件培养基诱导,诱导后细胞通过表皮细胞标志物CK19以及CK10染色鉴定。结果与结论:胎盘间充质干细胞表面抗原CD29、CD44、CD105阳性,CD34、CD45、CD106以及HLA-DR阴性。诱后细胞形态明显改变,表皮细胞标志物CK19以及CK10免疫荧光染色阳性,提示胎盘间充质干细胞在体外具有分化为皮细胞的能力,可以作为皮肤组织工程和干细胞研究的一种有效来源。

BACKGROUND：The pervious study has showed that placenta mesenchymal stem cells（PMSCs） can differentiate into bone,cartilage,and muscle and adipose mesenchymal stem cells.OBJECTIVE：To investigate the differentiation of PMSCs into epidermal cells in vitro.METHODS：PMSCs were isolated and cultured and the surface antigen was detected by flow cytometry.RESULTS AND CONCLUSION：Isolated PMSCs were positive for the markers CD105,CD29 and CD44 and negative for typical hematopoietic and endothelial markers CD34,CD45,CD106 and HLA-DR.The morphology of induced cells was obviously changed.Immunohistochemistry stain analysis showed positive for epidermal-specific markers like CK19 and CK10.The PMSCs have the capability to differentiate into epidermal cells in vitro.The placenta may prove to be a useful source of mesenchymal stem cells in skin tissue engineering and stem cells study.