2,5-己二酮染毒大鼠血清肌酸激酶、乳酸脱氢酶活性及髓鞘碱性蛋白含量的变化

Changes of the activities of creatine kinase,lactate dehydrogenates and the levels of myelin protein in HD-treated rats

目的研究2,5-己二酮染毒大鼠血清中肌酸激酶(creatine kinase,CK)、乳酸脱氢酶(lactatedehydrogenates,LDH)活性及髓鞘碱性蛋白含量(myelin basic protein,MBP)的变化,探讨它们作为正己烷中毒性周围神经病生物标志物的可能性。方法 Wistar雄性大鼠48只,随机分为对照组、低剂量染毒组和高剂量染毒组,每组16只。对照组给予生理盐水腹腔注射,低剂量和高剂量染毒组分别给予200和400 mg/kg 2,5-己二酮(2,5-hexanedione,HD)腹腔注射,连续6周。观察动物体重、神经行为学指标变化,实验结束后取腓肠肌做病理检查,取坐骨神经做电镜观察,测定血清CK、LDH活性和MBP含量。结果 HD染毒6周后,染毒大鼠出现步态异常,腓肠肌病理提示神经源性肌肉萎缩,坐骨神经电镜结果显示轴突变性。与对照组相比,200和400 mg/kg染毒组大鼠血清CK活性分别下降19.72%和54.37%,差异有统计学意义(P<0.05);与对照组相比,400 mg/kg染毒组大鼠血清MBP含量增加34.82%,差异有统计学意义(P<0.05);而LDH活性变化无统计学意义(P>0.05)。结论 2,5-HD中毒性周围神经病大鼠血清CK活性降低、MBP含量升高,它们有可能成为正己烷中毒性周围神经损害的早期生物标志物。

Objective To investigate changes of the activities of creatine kinase（CK） ,lactate dehydrogenates（LDH） and the level of myelin protein （MBP） in HD-treated rats for seeking potential biomarkers of peripheral nerve injury. Methods Totally 48 health Wistarrats were randomly divided into control group, low and high doses of 2, 5-hexanedione （HD） groups. The control group was injected physiological saline; 2,5-HD groups were potentially injected 2,5-HD with the dose of 200 mg/kg,400 mg/kg for one time per day,5 days per week, respectively. All three groups had been treated five times per week for six weeks. Body weight and gait score were measured per week. After the experiment gastrocnemius muscles and sciatic nerve were dissected for the pathological examination, and serum was taken to study levels of CK, LDH and MBP. Results Compared with the control group 2, 5-HD groups＇ weight decreased significantly and abnormal gaits were observed. There were neurogenic muscular atrophy in high dose 2, 5-HD group according to result of pathological examination and axonal degeneration according to transmission electron microscopy. Serum CK activity decreased by 19.72% and 54.37% （ P 〈 0. 05 ） ; Serum myelin basic protein levels increased by 34.82% （P 〈 0.05）. Conclusion After exposure to 2, 5-HD, Rats＇ serum CK activity decreased and serum myelin basic protein levels increased. The activity of CK and quantity of MBP may be candidate biomarkers for n- hexane poisoning.