维药买朱尼含药血清影响大鼠关节软骨细胞基质金属蛋白酶1及其组织抑制因子1的表达

Effects of Uygur medicine Maizhuni medicated serum on the expression of matrix metalloproteinase 1 and tissue inhibitor of metalloproteinase 1 in rat chondrocytes

背景:基质金属蛋白酶1及其组织抑制因子1的失衡是骨关节炎软骨降解的关键。目的:观察维药买朱尼对白细胞介素1β诱导的大鼠关节软骨细胞基质金属蛋白酶1及其组织抑制因子1表达的影响。方法:从1周龄SD大鼠关节中分离培养原代软骨细胞,鉴定后选用第2代细胞随机分为正常对照组、模型组、买朱尼组。模型组和买朱尼组用10μg/L的白细胞介素1β干预24h后,买朱尼组在此基础上加入体积分数10%的买朱尼含药血清,模型组加入正常大鼠血清,继续培养12,24,48h进行实验。结果与结论:各组细胞培养48h,RT-PCR检测发现体积分数10%的买朱尼含药血清可上调软骨细胞基质金属蛋白酶组织抑制因子1mRNA的表达同时抑制基质金属蛋白酶1mRNA的表达,但此作用在细胞培养的24,36h时不明显。同时免疫荧光细胞化学染色也显示买朱尼含药血清可促进软骨细胞基质金属蛋白酶组织抑制因子1的表达。说明买朱尼可以调节白细胞介素1β诱导的大鼠关节软骨细胞基质金属蛋白酶1及其组织抑制因子1的表达,且此作用具有时间依赖性。

BACKGROUND： Imbalance of matrix metatloproteinase 1 and its tissue inhibitor is the key of articular cartilage degradation in osteoarthritis. OBJECTIVE： To explore the effect of Uygur medicine Maizhuni on the expression of metalloproteinase 1 and tissue inhibitor of metalloproteinase 1 in rat articular cartilage cells induced by interleukin-β. METHODS： Primary chondrocytes were isolated from one week old SD rats, and then identified. Chondrocytes of passage 2 were randomly assigned into control group, model group and Maizhuni group. Chondrocytes in model group and Maizhuni group were treated with 10 μg/L interleukin-lβ for 24 hours. After that, chondrocytes in Maizhuni group and model group were further cultured for 12, 24 and 48 hours in addition with 10% Maizhuni medicated serum and rat serum, respectively. RESUILS AND CONCLUSION： According to RT-PCR results at 48 hours after cultivation, 10% Maizhuni medicated serum increased the mRNA expression of matrix metalloproteinase 1 tissue inhibitor and suppressed the mRNA expression of matrix metal^oproteinase 1 in chondrocytes. But the effect was not obvious at 24 and 36 hours after cultivation. Immunofluorescence and cytochemical staining results showed that Maizhuni medicated serum increased the expression of matrix metalloproteinase 1 tissue inhibitor in chondrocytes. These findings indicate that Maizhuni can regulate the expression of metalloproteinase 1 and its tissue inhibitor in rat chondrocytes induced by interleukin-1β in a time-dependent manner.