香烟提取物损伤人脐静脉血管内皮细胞及阿托伐他汀的干预

Human umbilical vein endothelial cells damaged by cigarette smoke extract and intervention of atorvastatin

背景:吸烟是诸多血管缺血性疾病如动脉硬化闭塞、血栓闭塞性脉管炎等疾病的重要危险因素,但其确切机制尚不清楚。目的:探讨香烟提取物对人脐静脉内皮细胞的影响以及阿托伐他汀对该作用的干预。方法:将体外培养的人脐静脉内皮细胞分为3组,分别以含体积分数10%胎牛血清的DMEM培养基对照组、体积分数10%胎牛血清+10%香烟提取物的DMEM培养基、含体积分数10%胎牛血清+10%香烟提取物+10μmol/L阿托伐他汀的DMEM培养基培养。结果与结论:香烟提取物组人脐静脉内皮细胞形态较对照组不规则,存活率、细胞上清液中的一氧化氮水平和细胞一氧化氮合酶活性降低(P<0.05);香烟提取物+阿托伐他汀组使人脐静脉内皮细胞形态改善,存活率、细胞上清液中的一氧化氮水平和细胞一氧化氮合酶活性升高(P<0.05)。说明阿托伐他汀能拮抗香烟提取物对血管内皮细胞的损伤作用,其机制可能与内皮细胞的生长、一氧化氮合成酶活性及一氧化氮释放有关。

BACKGROUND： Smoking is an important risk factor for many vascular ischemic diseases, such as arterial occlusive and thromboangiitis obliterans. However, the exact mechanism behind this is unclear. OBJECTIVE： To investigate the effects of cigarette smoke extract on human umbilical vein endothelial cells and the intervention effect of atorvastatin during this process. METHODS： Human umbilical vein endothelial cells were cultured in vitro and divided into three groups, control group, cigarette smoke extract group and cigarette smoke extract plus atorvastatin group. Control group were cultured in DMEM medium containing 10% fetal bovine serum. Cigarette smoke extract group were cultured in DMEM medium containing 10% fetal bovine serum and 10% cigarette smoke extract. Cigarette smoke extract plus atorvastatin group were cultured in DMEM medium containing 10% fetal bovine serum, 10% cigarette smoke extract and 10 pmol/L atorvastatin. RESULTS AND CONCLUSION： Compared with control group, the cell morphology in cigarette smoke extract group was much irregular, the survival rate, the nitric oxide level in cell supernatant and the enzyme activity of nitric oxide synthase was decreased （P 〈 0.05）. The cell morphology in cigarette smoke extract plus atorvastatin group was improved, the survival rate, the nitric oxide level in cell supernatant and the enzyme activity of nitric oxide synthase was increased （P 〈 0.05）. It indicates that atorvastatin can protect vein endothelial cells from the damage effects of cigarette smoke extract. The mechanism may be related to the endothelium cell growth, the enzyme activity of nitric oxide synthase and the release of nitric oxide.