慢性肺动脉血栓栓塞动物模型的建立

Construction of a chronic pulmonary thromboembolism animal model

背景:目前国内外接近人体生理学的慢性肺动脉血栓栓塞的动物模型较少见。目的:采用自体血栓反复注射法建立慢性肺动脉血栓栓塞的动物模型。方法:将家兔随机分为栓塞组和假栓塞组,栓塞组家兔肺动脉多次注入自体血栓,假栓塞组用生理盐水替代血栓。结果与结论:栓塞组干预4周后解剖肺动脉并经病理检测可发现有血栓机化,肺动脉CT造影均可见局部肺动脉截断征,以及炎症、梗死、胸膜增厚等间接征象,肺动脉解剖及病理均可发现实验兔肺动脉血栓形成机化及慢性炎症改变。结果证实,采用兔自体血栓反复注射法可成功建立慢性肺血栓栓塞动物模型。

BACKGROUND： Studies on chronic pulmonary thromboembolism animal models close to the human physiology are rare at home and aboard. OBJECTIVE： To establish a chronic pulmonary thromboembolism animal model by repeated injecting autelogous blood clot. METHODS： Rabbits were randomly assigned into embolism group and mock embolism group. Embolism rabbits were repeatedly injected with autologous blood clot through the arteries. Mock embolism rabbits were injected with saline instead. RESULTS AND CONCLUSION： According to the anatomy of pulmonary artery and pathological examination, there was thrombus organization in the 4t＂ week after autologous blood clots injection. CT angiography of pulmonary artery represented signs of local pulmonary artery truncation and some indirect signs, such as inflammation, infarction and pleural thickening. Pulmonary anatomy and pathological examination discovered pulmonary artery thrombus organization and chronic inflammation in the experimental rabbits. These findings demonstrate that chronic pulmonary thromboembolism rabbit model is successfully constructed by repeated injection of autologous blood clot.