线栓法制备SD大鼠局灶性脑缺血再灌注损伤模型

Preparation of Sprague-Dawley rat models of focal cerebral ischemia-reperfusion injury by suture method

背景:线栓法制备大鼠局灶性脑缺血再灌注损伤模型的方法尚无统一标准,寻找一种操作简单,稳定性好,成功率高的局灶性脑缺血再灌注模型方法在缺血性脑病研究中有重要意义。目的:采用线栓法制备SD大鼠局灶性脑缺血再灌注损伤模型,分析模型制作的成功率和稳定性。方法:对传统ZeaLonga模型制作方法进行改进,选用鱼线作为栓线,结扎颈外动脉及颈总动脉近心端,不剪断颈外动脉,不结扎翼腭动脉,栓线通过颈总动脉进入大脑中动脉造成缺血,拔出线栓形成再灌注。结果与结论:建模型时间均在20min内完成,建模后大鼠神经功能缺损明显,红四氮唑染色示脑梗死区苍白,病理学检查有典型的病理表现,模型成功率为75%,在制作过程中易出现蛛网膜下腔出血、脑缺血不完全等问题。结果表明,线栓法制备SD大鼠局灶性脑缺血再灌注损伤模型的方法成功率高,缺血效果明确,时间短,是一种简单方便的制作局灶性脑缺血再灌注损伤模型的方法。

BACKGROUND： There have been no unified standards regarding suture method applied for preparation of focal cerebral ischemia-reperfusion injury models in rats. It is of great significance to search a simple, stable and successful method to prepare focal cerebral ischemia-reperfusion injury models in study of ischemic cerebral disease. OBJECTIVE： To prepare focal cerebral ischemia-reperfusion injury models in Sprague-Dawley rats using suture method and to analyze the success rate and stability of model preparation. METHODS： The conventional Zea Longa model preparation method was modified. Precisely, fishing line was used as the suture line. The proximal parts of external carotid artery and internal carotid artery were ligated. The external carotid artery was not cut off and the pterygopalatine artery was not ligated. The suture line was advanced into the middle cerebral artery via the common carotid artery to cause ischemia and reperfusion. RESULTS AND CONCLUSION： Middle cerebral artery occlusion was performed within 20 minutes. After model preparation, rat neurological functions were obviously impaired. Red tetrazoline staining showed that cerebral infarction region was pale. Pathological examination showed typical pathological manifestation. The success rate of model preparation was 75%. During model preparation, subarachnoid hemorrhage and incomplete cerebral ischemia may occur. These findings suggest that the suture method applied for preparation of focal cerebral ischemia reperfusion injury models in Sprague-Dawley rats is a simple method with high success rate, identified ischemic effects and short operation time.