角膜穿通损伤合并海水浸泡模型兔角膜组织病理学及房水白细胞介素6的变化(英文)

Corneal histopathological changes and interleukin-6 level in aqueous humor of a rabbit model of penetrating corneal trauma combined with seawater immersion

背景:由于海水特殊的理化性质,海上创伤后细胞的病理改变常有特殊性。目的:观察兔角膜爆炸穿通伤合并海水浸泡角膜组织病理学改变及房水白细胞介素6水平变化。方法:取成年健康灰兔16只,单支鞭炮致兔角膜爆炸伤,于角膜中周部做长3mm全层切口。右眼为实验侧,左眼为对照侧。实验侧将海水通过角膜切口注入前房,海水持续冲洗眼表30min。对照侧使用生理盐水。结果与结论:光镜观察显示实验侧造模后1、2和3d角膜上皮细胞大片坏死脱落,基质层水肿明显伴大量炎细胞浸润。对照侧1、2d角膜病理改变同实验组,但程度较轻,造模后3、5d局部病理改变明显减轻。实验组造模后第1、2、3d房水中白细胞介素6水平明显高于对照侧(P<0.05)。表明海水组伤情较对照组明显加重,提示海水可能是重要的致伤因素之一。

BACKGROUND： Following trauma caused by seawater, cells often exhibit special pathological changes because of the special physico-chemical properties of seawater. OBJECTIVE： To observe corneal histopathological changes and interleukin-6 level in aqueous humor of a rabbit model of penetrating corneal trauma combined with seawater immersion. METHODS： The rabbit eye models of penetrating corneal trauma caused by firecrackers were established in 16 adult healthy grey rabbits. A 3-mm whole-layer incision was made in the cornea. The right eyes served as experimental sides and the left eyes served as controls. Seawater was injected into the aqueous humor of the right eyes via the corneal incision. The eye surface was flushed with seawater for 30 minutes. Physiological saline was used for the left eyes. RESULTS AND CONCLUSION： Optical microscopy results showed that at 1, 2, 3 days after model establishment, corneaF cells on the experimental side exhibited severe necrosis and abscission, obvious swelling of substantia propria layer complicated by cellular infiltration. At 1 and 2 days after model establishment, the pathological changes on the control side were the same as the experimental side, but they were mild, but at 3 and 5 days, they were obviously alleviated. At 1, 2, 3 days after model establishment, interleukin-6 level in aqueous humor was significantly higher on the experimental side than on the control side （P 〈 0.05）. These findings suggest that the degree of injury on the experimental side was more severe than that on the control side, indicating that seawater may be an important causative factor of corneal injury.