邻苯二甲酸二丁酯诱导孕鼠尿道下裂模型后仔鼠睾丸细胞过氧化物酶6的表达

Expression of peroxidase 6 in fetal rat testicular cells in pregnant rat hypospadias model induced by di-n-butyl phthalate

前期实验发现在邻苯=甲酸二丁酯孕期暴露诱导的仔鼠睾丸发育中蛋白过氧化物酶6表达显著增高。目的：进一步验证邻苯二甲酸二丁酯胚胎期暴露对胎鼠睾丸蛋白过氧化物酶6表达的影响。方法：妊娠14～18d按800mg，（kg·d）邻苯二甲酸二丁酯染毒孕鼠，妊娠21d取出胚胎大鼠睾丸，提取蛋白，通过WeslembIotting及免疫组织化学方法检测睾丸细胞过氧化物酶6的表达，以正常雄性仔鼠睾丸为对照。结果与结论：WeslembIot帅g检测显示，实验组过氧化物酶6的相对表达量为0．205±0．020，对照组为0．110±O．020，实验细明显高于对照组（P〈0．05）。免疫组织化学方法发现过氧化物酶6主要定位于胎鼠睾丸Leydig细胞中。说明过氧化物酶6可能通过发挥抗氧化作用及刺激Leydig细胞增殖功能抵抗邻苯二甲酸=!丁酯的毒性作用，其功能有待进一步研究。

BACKGROUND： Previous study shows that expression of peroxidase 6 in fetal rat testis increase significantly induced by in utero exposure to di-n-butyl phthalate. OBJECTIVE： To further investigate the effect of prenatal di-n-butyl phthalate exposure on peroxidase 6 expression. METHODS： Rats of 14 to 18 days of pregnancy were exposed to di-n-butyl phthalate with the dose of 800mg / （kgod）. Testes were harvested from the fetal rats from gestational day 21 and peroxidase 6 was extracted. The expression of peroxidase 6 was detected and analyzed by western blot and immunohistochemical methods. RESULTS AND CONCLUSION： According to western blot, the expression of peroxidase 6 in experiment group （0.205＋0.020） was significantly higher than that in the control group （0.110 ＋ 0.020, P 〈 0.05）. According to immunohistochemistry, peroxidase 6 was mainly localized in Leydig cells of fetal rat testes. These findings indicate that peroxidase 6 may antagonize di-n-butyl phthalate toxicity by antioxidation and stimulating the proliferation of Leydig cells. The function of peroxidase 6 needs further research.