早期注射丹参酮ⅡA磺酸钠可抑制兔耳增生性瘢痕

Early application of sodium tanshinoneⅡA sulfonate can depress hypertrophic scar of rabbit ear

背景:丹参酮ⅡA磺酸钠具有抗氧化、抑制纤维化等作用。目的:观察丹参酮ⅡA磺酸钠早期局部注射对兔耳增生性瘢痕的影响。方法:于兔耳腹侧面切除全层皮肤及软骨膜建立瘢痕模型,造模后28d,分别在创面瘢痕内注射0.05,0.1,0.2mg的丹参酮ⅡA磺酸钠或生理盐水,每周注射1次,共3周。结果与结论:丹参酮ⅡA磺酸钠注射3周,兔耳瘢痕增生较轻,瘢痕厚度变薄;苏木精-伊红染色见瘢痕组织中胶原纤维减少,血管数目减少;Masson染色检测见瘢痕组织胶原纤维分布面积减少;电镜下瘢痕组织成纤维细胞数量减少,体积相对变小。说明早期注射丹参酮ⅡA磺酸钠对兔耳瘢痕增生有抑制作用。

BACKGROUND： Sodium tanshinone ⅡA sulfonate （STS） play effects on anti-oxidant, and depression of fibration. OBJECTIVE： To investigate the effect of early injection of STS on hypertrophic scar （HS） of rabbit ear. METHODS： Scar model were established by removing the skin and perichondrium in rabbits ears on facies ventralis. STS 0.05 mg, 0.1 mg, 0.2 mg （or 40pl normal saline, respectively, experimental groups）, normal saline 40 pL（control group） were injected into early rabbits ears scars, once a week, 3 weeks all together. RESULTS AND CONCLUSION： After 3 weeks STS injection, the hyperplasia and the thickness were decreased; the quantity of collagen fiber and vascular in scar tissue section was decreased in experimental group detected by hematoxylin-eosin staining. The collagen fiber area in scar tissue section was decreased by Masson staining and the amount and the volume of fibroblast were decreased under electron microscope. That indicated that early STS injection could depress HS in rabbits scar ears.