牡丹江地区汉族及朝鲜族高血压人群中G蛋白β3亚单位825C/T的多态性

Polymorphism of G protein β3 subunit 825C/T in Korean population and Han population with essential hypertension in Mudanjiang region

背景:G蛋白β3亚单位825C/T基因多态性与朝鲜族人群原发性高血压是否存在关系尚无报道。目的:研究牡丹江地区朝鲜族、汉族高血压患者C825T基因的多态性。方法:纳入2008-09/2010-08在牡丹江市朝鲜民族医院内科就诊的原发性高血压患者224例作为病例组,同时选取同时期的门诊体检的健康者196例作为对照。用PCR-RFLP检测G蛋白β3亚单位基因C825T多态性,观察TT,CT,CC基因型频率及等位基因频率。结果与结论:PCR-RFLP检测结果显示牡丹江地区朝鲜族高血压患者与朝鲜族健康人TT,CT,CC基因型频率差异无显著性意义(P>0.05);T,C等位基因频率差异有显著性意义(P<0.05)。而牡丹江地区汉族人群中TT,CT,CC基因型分布在高血压人群和正常人群差异有显著性意义(P<0.05),而T,C等位基因频率差异无显著性意义(P>0.05)。朝鲜族高血压组等位基因C的比例较高,而汉族高血压组等位基因T的比例较高,存在民族异质性。提示G蛋白β3亚单位基因C825T多态性与牡丹江地区朝鲜族高血压无关联,但其是牡丹江地区汉族高血压的危险因素。

BACKGROUND： Whether there is a relationship between the gene polymorphisms of G-protein [33 subunit 825 C/T and the primary hypertension of Korean population remains unanswered. OBJECTIVE： To explore the gene polymorphisms of C825T in Korean population and Han population with essential hypertensive in Mudanjiang. METHODS： A total of 224 essential hypertensive patients receiving medical serving in the Internal Medicine Department of Koreans Hospital of Mudanjiang City were selected as hypertension group. A number of 196 healthy people underwent physical examinations in the corresponding period at the clinic were selected as normotensive group. PCR-RFLP method was used to measure the polymorphism of G-protein ~3 subunit C825T. Genotype frequencies and allele frequencies of TT, TC and CC were investigated. RESULTS AND CONCLUSION： PCR-RFLP results demonstrated that with respect to Korean population in Mudanjiang, there was no significant difference in the genotype frequencies of TT, TC and CC between the hypertension group and normotensive group （P 〈 0.05）, and there was significant difference in the allele frequencies of T and C between these two groups （P 〈 0.05）. With respect to Hart population in Mudanjiang, there was significant difference in the genotype frequencies of TT, TC and CC between the hypertension group and normotensive group （P 〈 0.05）, but no significant differences in allele frequencies of T and C between these two groups （P 〉 0.05）. Allele frequency of C in hypertension group of Korean population was higher while the allele frequency of T was higher in hypertension group of Han population. There was a difference between nations. These findings indicate that the gene polymorphisms of G-protein 133 subunit C825T is not related to hypertension of Korean population in Mudanjiang, but is a risk factor of hypertension in Mudanjiang Han population.