乳腺癌中RRIG1 mRNA的表达及意义

Expression of RRIG1 gene mRNA in breast carcinoma and its clinicopathologic significance

目的探讨维甲酸受体诱导基因1(retinoic receptor-induced gene 1,RRIG1)在人乳腺癌组织中的表达及其与患者临床病理参数的关系。方法以GAPDH为内对照采用实时荧光定量逆转录聚合酶链反应法(real time quantitative reverse transcrip-tion polymerase chain reaction,RQ RT-PCR)检测76例新鲜乳腺浸润性导管癌组织5、例导管原位癌组织和20例乳腺良性增生组织中RRIG1 mRNA的表达水平,并分析其与患者临床病理参数的关系。结果乳腺导管原位癌、浸润性导管癌组织中RRIG1 mRNA相对表达水平(3.5±2.1、6.7±4.1)明显低于良性增生组织(10.2±6.5),差异具有统计学意义(P=0.004,P=0.036);乳腺浸润性导管癌中淋巴结转移组RRIG1 mRNA相对表达水平(5.2±3.1)明显低于无淋巴结转移组(7.48±4.5),两组之间差异具有统计学意义(P=0.023)。结论 RRIG1可能作为一种新的抑癌基因参与乳腺癌的发生、浸润和转移,对乳腺癌早期诊断和预后评估具有重要的参考价值。

Purpose To investigate the expression of RRIG1 mRNA and their relationships with clinicopathologic characters in breast carcinoma. Methods Real time quantitative reverse transcription polymerase chain reaction （ RQ RT-PCR） technique was applied for detecting RRIG1 mRNA expression in 76 cases of breast carcinoma, 5 cases of ductal carcinoma in situ and 20 cases of breast benign lesions. Results The level of mRNA expression of RRIG1 gene was significantly higher in breast benign 1 esions （ 10. 2 _＋ 6. 5 ） than in ductal carcinoma in situ（3.5 ~ 2. 1 ） and invasive breast carcinoma（6. 7 -＋ 4. 1 ） （P = 0. 004 and 0. 036, respectively）. RRIG1 mRNA expression is significantly lower in lymph node positive （5.2 ＋ 3.1 ） than in non lymph node negative cases （7.48 ~ 4. 5 ） （P = 0. 023 ）. Conclusions RRIG1 may play a important role in disease occurrence and progression of breast carcinoma, RRIG1 may be a new bio- marker for early diagnosis and prognosis in breast cancer.