阿霉素处理的肿瘤抗原致敏树突状细胞的抗肿瘤免疫研究

Efficient Induction of a U14-specific Anti-tumor Response by Dendritic Cells Sensitisized with ADM-U14 Antigen

研究表明化疗药物作用于肿瘤细胞后可有效激发免疫应答,这与肿瘤细胞的性质和化疗药物有关。该研究主要探讨阿霉素(adriamycin,ADM)处理小鼠宫颈癌U14细胞获得的肿瘤抗原致敏树突状细胞(dendritic cells,DCs)的免疫应答及对肿瘤的杀伤效应。分别应用ADM和反复冻融法处理小鼠宫颈癌U14细胞,取其离心上清液,致敏小鼠骨髓来源的DC,观察DC诱导的淋巴细胞增殖反应和细胞毒性T淋巴细胞(cytotoxic T lymphocyte,CTL)对宫颈癌细胞的细胞毒效应。结果显示:ADM处理的U14细胞抗原致敏后的DC组所激发和扩增的T细胞数及对宫颈癌细胞的杀伤效果显著高于对照组(P<0.05)。因而提示ADM处理的肿瘤抗原能有效地致敏DC并产生抗肿瘤免疫效应。

Studies have shown that tumor cells treated with chemotherapy drugs can effectively stimulate the immune response, which is dependent on the nature of the tumor cells and chemotherapy drugs. We reported bone marrow derived-DCs were sensitized with the culture supernatants from mice ADM-U14 after centrifugation.Then, the lymphocyte proliferation and the cytotoxic effects induced by the sensitized DCs were evaluated using MTT assays. We found the number of lymphocyte proliferation and the killing effects on mice U14 cells in the sen-sitized DCs group were much higher than those in the control group. Thus our results suggest mice ADM-U14 an- tigen can effectively sensitize DCs, and activate T lymphocytes proliferation and produce cytotoxic T lymphocytes （CTLs） to kill tumor cells in vitro.