乳腺癌中MUC2和MMP-9的表达及其临床意义

Expression of MUC2 and MMP-9 in Breast Carcinoma and Its Clinical Significance

目的:探讨MUC2和MMP-9在乳腺癌中的表达及其与临床病理特征之间的关系。方法:应用逆转录-聚合酶链反应(RT-PCR)方法检测68例乳腺良性疾病和186例乳腺癌中MUC2和MMP-9基因的表达,免疫印迹法观测MUC2和MMP-9蛋白表达情况。分析两者与乳腺癌临床病理特征之间的关系及两者的相关性。结果:MUC2和MMP-9mRNA在乳腺癌中的表达明显高于乳腺良性疾病组。乳腺良性疾病组织中MUC2和MMP-9mRNA的表达率分别为2.9%(2/68)、13.2%(9/68),均显著低于癌组织39.8%(53/186)、73.1%(136/186)(P<0.05)。类似于基因表达,MUC2和MMP-9蛋白在乳腺癌中的表达相对较高。MUC2和MMP-9基因在乳腺癌中的表达与病理类型、淋巴结转移相关(P<0.05),MMP-9表达还与肿瘤大小和组织学分级有关(P<0.05),两者均与患者年龄无关(P>0.05)。乳腺癌组织中MUC2和MMP-9mRNA的表达呈负相关(r=-0.814,P<0.001)。结论:MUC2和MMP-9联合检测可作为诊断乳腺疾病的指标之一,为乳腺癌诊断和治疗提供参考。

Objective： To investigate the MUC2 and MMP-9 expression in breast carcinoma tissue and to analyze its relationships with clinical characteristics. Methods： Reverse transcription PCR was applied to detect the MUC2 and MMP-9 mRNA expression in benign breast diseases and breast cancer. The expression levels of MUC2 and MMP-9 were measured by Western blot. The correlations of MUC2 and MMP-9 with clinical characteristics and the relationship between these two proteins were analyzed. Results： The grayscale bands of the MUC2 and MMP-9 mRNAs in breast tumors were significantly higher than those in benign breast diseases. The expression levels ofMUC2 mRNA in benign breast diseases and breast carcinoma were 2.9% （ 2/68 ） and 39.8% （ 53/186 ）, respectively （ P 〈 0.05 ）, whereas those of MMP-9 mRNA were 13.2% （ 9/68 ） and 73.1% （ 136/186 ）, respectively （ P 〈 0.05 ）. Moreover, the MUC2 and MMP-9 expression was significantly lower in benign diseases than in breast tumors. The MUC2 and MMP-9 mRNA expressions were correlated with the tumor type and lymph-node metastasis of breast carcinoma, respectively （ P 〈 0.05 ）. In addition, the MMP-9 mRNA expression was correlated with histological grade and tumor size. However, no significant associations were found between gene expression and patient age （ P 〉 0.05 ）. The MMP-9 expression had a significantly inverse correlation with MUC2 （ r = -0.814; P 〈 0.001 ）. Conclusion： The MUC2 and MMP-9 expression may be indicators of breast cancer. They may be suitable indicies for diagnosis and treatment of breast carcinoma.