紫草组分的抗肿瘤活性及其凋亡机制研究

Study on antitumor activities and its mechanisms of Lithospermum components

目的研究紫草组分的体外抗肿瘤作用及其作用机制。方法用MTT法、流式细胞仪,观察1.25～10.00μg.mL-1紫草组分对B16F10及HepG2细胞作用24 h的生长抑制作用及凋亡诱导作用;用激光共聚焦显微镜,观察药物的细胞分布;用Western-blot法检测细胞凋亡相关蛋白分子的变化。结果紫草组分能够抑制HepG2和B16F10细胞的增殖并诱导其早期凋亡,并呈浓度与时间依赖性。药物主要分布于细胞质中,在给药后30 min,给药组的p-JNK与p-p38分子明显增加。结论紫草组分可抑制HepG 2和B16F10细胞的增殖,并诱导细胞凋亡。

Objective To investigate the effects of Lithospermum components of Arnebia euchroma（Royle） Johnst on proliferation inhibition and apoptosis induction on human hepatoma HepG2 cells and B16F10 cells and its possible mechanism.Methods The proliferation inhibition was measured by MTT assay.FCM（flow cytometer） method was employed to detect the degree of apoptosis.Confucal was used to detect the distribution of the components in B16F10 cells.And western-bot was used to observe the change of the apoptosis-related proteins.Results Lithospermum components of Arnebia euchroma（Royle）Johnst inhibited HepG2 cells and B16F10 cells′ growth in a time-and dose-dependent manner.With the increased concentration,the apoptosis degree increased after treated with drug for 24 hours.The components mainly distributed in the cytoplasmic,and p-JNK,p-p38 in the cells increased after treated with the components.Conclusion Lithospermum components of Arnebia euchroma（Royle）Johnst can inhibit the growth and induce the apoptosis of HepG2 cells and B16F10 cells significantly.