NDRG1/c-kit在NB4细胞的表达

Expression of NDRG1/c-kit in NB4 cells

目的观察NDRG1和c-kit在急性早幼粒细胞白血病NB4细胞株的表达,探讨全反式维甲酸(ATRA)和三氧化二砷(As2O3)诱导NB4细胞分化的作用机制。方法采用1.0μmol/L ATRA、0.1μmol/L和1.0μmol/L As2O3分别诱导NB4细胞。台盼蓝染色计算细胞生长率,吉姆萨染色观察细胞形态学变化,应用RT-PCR检测NDRG1和c-kit的表达。结果 ATRA明显上调了NDRG1 mRNA的表达,同时使c-kit mRNA的表达下调。As2O3也诱导了NDRG1在NB4的表达,但对c-kit没有作用。结论 ATRA和As2O3均可诱导NB4细胞分化,但其作用机制是通过调节不同的分子信号途径实现的。

Objective To investigate the mechanisms of differentiation in acute promyelocytic leukemia （APL） cell line NB4 treated with ATRA and As203 by the observation of NDRG1 （N-mye downregulated gene 1 ） and c-kit gene expression. Methods 1.0 μmol/L ATRA, 0. 1 μmol/L and 1.0 p, mol/L AsaO3 were used to induce NB4 cells respectively. Cells were counted with trypan blue exclusion. Cell morphological features were observed by Giemsa staining. The expression of NDRG1 and c-kit gene was examined by RT-PCR. Results ATRA induced the NDRG1 mRNA upregulation in NB4 cells, and c-kit mRNA was down regulated. However, As2O3 induced the NDRG1 expression in NB4 cells, but the effect was not seen on c-kit. Conclusion ATRA and As2O3 can induce NB4 differentiation by the mediate distinct signal path-way.