邻苯二甲酸（2-乙基己基）酯对妊娠大鼠的影响及锌保护作用的研究

Study on the effect of di-（2-ethylhexyl） phthalate on pregnant rats and the protection of zinc against it in pregnancy

目的探讨邻苯二甲酸（2-乙基己基）酯（DEHP）对大鼠妊娠结局的影响及锌对其影响的保护作用。方法将50只雌鼠随机分为DEHP组（50mg^-1kg·d^-1）、DEHP＋锌组（含1．2mg锌的葡萄糖酸锌＋50nag·kg^-1·d^-1DEHP）、空白组（灌注1ml生理盐水）、玉米油组（灌注1ml玉米油）、锌组（含1．2mg锌的葡萄糖酸锌1m1）5组，每组10只，孕前7d开始每日给予相应药物灌胃，直至孕19d处死孕鼠，取出胎鼠。测量孕鼠体质量、各脏器质量、活胎鼠数、活胎鼠体质量及胎盘质量。结果孕鼠体质量及肾、脾、脑、心脏等脏器质量指数各组问两两比较，差异均无统计学意义（P〉0．05）。孕鼠肝、子宫及卵巢质量指数，空白组分别为（4．4±0．7）％、（1．264-0．09）％、（0．083±0．009）％，玉米油组分别为（4．5-4-0．6）％、（1．29±0．10）％、（0．084±0．008）％，锌组分别为（4．4±0．4）％、（1．26±0．08）％、（0．084±0．009）％，DEHP组分别为（5．4±1．0）％、（1．11±0．08）％、（0．074±0．012）％，DEHP＋锌组分别为（4．4±1．0）％、（1．28±0．10）％、（0．082±0．007）％，DEHP组与其他4组两两比较，差异均有统计学意义（P〈0．05），其他4组间两两比较，差异均无统计学意义（P〉0．05）。活胎鼠数、活胎鼠体质量及胎盘质量，空白组分别为（12．8±2．7）只、（6．03±0．16）g、（1．00±0．03）g，玉米油组分别为（13．6±3．1）只、（6．07±0．20）g、（1．00-4-0．04）g，锌组分别为（13．3±3．1）只、（6．16±0．18）g、（1．00±0．05）g，DEHP组分别为（9．24-4．1）只、（4．03±0．09）g、（0．95±0．03）g，DEHP＋锌组分别为（12．1±2．9）只、（6．09±0．17）g、（0．994-0．03）g，DEHP组与其他4组两两比较，差异均有统计学意义（P〈0．05），其他4组间两两比较，差异均无统计学意义（P〉0．05）。结论妊娠期DEHP的暴露可对孕鼠及胎鼠造成损伤，预先给予锌干预可减轻DEHP暴露对妊娠的影响，保护妊娠过程。

Objective To investigate the effect of di-（ 2-ethylhexyl ） phthalate （ DEHP ） on pregnant rat and the protection of zinc. Methods Fifty rats were randomized equally into 5 groups consisting of blank（ given 1 ml 0. 9% sodium chloride）, corn oil （given I ml corn oil） , zinc （given 1 ml zinc gluconate including 1.2 mg zinc） , DEHP （given 50 mg· kg^-1· d^-1 DEHP）and DEHP ＋ zinc （given 50 mg. kg^-1· d^-1 DEHP ＋ zinc gluconate）. At the beginning of the experiment （about 7 d before pregnancy） , the female rats were administered with corresponding drugs everyday. The pregnant rats were killed and the fetal rats were removed on 19th day. The following results in each group were recorded： the body weight and the organic weight of the female rats, the number and the weight of fetus rats and the placental weight. Results The weight and the coefficient of female rats＇ kidney/body, spleen/body, brain/ body, and heart/body in DEHP group compared with other groups were not statistical significance （all P 〉0. 05）. The coefficient of female rats＇ liver/body, uterus/body, and ovary/body of blank group were （ 4. 4 ± 0. 7 ） % , （ 1.26 ±0. 09 ） % , （ 0. 083 ±0. 009 ） % respectively, corn oil group were （ 4. 5 ± O. 6 ） % , （1.29±0.10）%, （0.084±0.008）%, zinc group were （4.4±0.4）%, （1.26 ＋0.08）%, （0.084±0.009）%, DEHP group were （5.4±1.0）%, （1.11±0.08）%, （0.074±0.012）%, andDEHP＋zinc group were （4.4 ± 1.0）% , （1.28 ±0. 10）% , （0.082 ＋0.007）% ; in DEHP group the coefficient of female rats＇ liver/body, uterus/body, and ovary/body compared with other groups was statistical significance （P 〈 0. 05 ）. The fetal quantity, fetal weight and placental weight of female rats of blank group were 12. 8 ＋ 2. 7, （6.03 ±0. 16） g, （1.00 ±0. 03） g respectively, eorn oil group were 13.6 ±3.1, （6. 07 ±0. 20） g, （1.00±0.04） g, zine group were 13.3 -＋3.1, （6. 16＋0.18） g, （1.00＋0.05） g, DEHP group were 9.2±4.1, （4.03 ±0.09） g, （0.95±0.03） g, and zinc ＋ DEHP group were 12.1 ＋2.9, （6.09 ±0. 17） g, （0. 99 ±0. 03） g. In DEHP group the fetal quantity, fetal weight and placental weight of female rats compared with other groups were statistical significance （ P 〈 0. 05 ）. Conclusion DEHP can damage female rats and fetal rats in gestation perioal Zinc supplied before pregnancy can relieve the influence by DEHP.