一种新型机械应变细胞加载装置的研制

Development of a novel stretch strain cell loading unit

目的研究开发一套具有自主知识产权的数控机械应变细胞加载装置,为细胞力学研究提供必要的研究手段。方法加载装置基于圆形基底形变技术,采用数字式测控系统和基于PC机平台的专用软件,实现对体外培养细胞加载牵张应变。采用MTT比色实验检测人牙周膜细胞在弹性硅橡胶细胞培养膜上的附着生长能力。采用该装置对体外培养人牙周膜细胞加载1%、10%、20%拉伸应变0.5、1和24 h,倒置相差显微镜观察细胞形态、排列的变化。结果数控机械应变细胞加载装置可对体外培养细胞加载不同强度、频率和时间的拉伸应变,具有输出应变范围大、精度高、操作方便、显示直观等优点。硅橡胶膜和对照细胞培养板接种细胞1、2、4、7、8 d后,MTT比色实验光吸收值之间无统计学差异(P>0.05),显示弹性硅橡胶膜具有良好的细胞附着生长能力。人牙周膜细胞加载10%、20%拉伸应变24 h后,细胞形态、排列发生改变,胞体呈长梭形,并成栅栏状平行排列,细胞长轴垂直于拉伸应变方向。结论数控机械应变细胞加载装置可有效地对体外培养细胞加载动态机械拉伸应变,为体外细胞力学研究提供了必要的研究手段。

Objective To develop a set of stretch strain cell loading unit with proprietary intellectual property rights so as to provide a necessary tool for the research of cellular mechanics. Methods Based on the deformation theory of round substrates, the stretch strain cell loading unit could apply the stretch strain to cultured cells in vitro by utilizing digital measuring-controlling system and computer software. MTT colorimetric test was adopted to evaluate the ability of human periodontal ligament cells （HPDLCs） to attach and grow on the silastic membrane. The cultured HPDLCs were loaded with ]%, 10% and 20% stretch strain for 0.5, ! and 24 h, respectively, by the loading unit, and an inverted phase contrast microscope was used to observe the morphology and realignment of HDPLCS. Results The stretch strain cell loading unit could apply the stretch strain of different magnitude, fre- quency and duration to cultured cells in vitro, with the advantage of a large range strain output, high precision, convenient operation and clear visual monitoring. When cells were seeded for ], 2, 4, ？, 8 d, respectively, there was no statistical difference between the MTT absorbance value of silastic membrane group and that of control group （P 〉0.05）, showing the good ability of cultured cells to attach and grow on the silastic membrane. After HPDLCs were loaded with 10% and 20% strain for 24 h, the morpholoqv and realiqnment of HPDLCs chanaed.and cells became spindle shaped, being parallel to each other with long axis perpendicular to the stretch vector. Conclusions The stretch strain cell loading unit is capable of loading the stretch strain to cultured cells effectively and provides a necessary method for cytomechanical research.