香精香料废水降解微生物的筛选及鉴定

Screening and Identification of Microorganisms for Degrading Essence and Spice

山东省某家香精香料厂废水中主要有机污染物是芳香族化合物,以叔丁基苯酚最难降解。为了筛选香精香料废水降解微生物,实验采集上述香精香料厂好氧曝气池的活性污泥为样本,以叔丁基苯酚为唯一碳源,进行筛选并分离出一株能降解废水的微生物。对该微生物的形态、生理生化特征进行鉴定,并对它的16S rDNA基因序列进行分析比对。结果表明,该菌株属于类球红细菌属(Rhodobacter sp.),适应偏盐性和适当芳香有机物的废水环境中生长并能有力地降解废水中的叔丁基苯酚。研究认为该菌株在有关废水处理方面的贡献难以估量。

It is aromatic compound which is mainly organic pollution that essence and spice industry wastewater in Shan dong, para-tertiary butyl phenol is very difficult degradation. In order to screen biodegradation microbiology in essence and spice industry wastewater, a strain it existing in para-tertiary butyl phenol was isolated from activated flavors and fragrances industry wastewater with mixed organic compounds as sample. According to thxonomic outline of the prokaryotes Bergey＇s Manual of Systematic Bacteriology （ the 8th version） and the Routine Germ Authenticates a Manual（ Edited by Dong Xiu-zhu, et al. ） that it was identified according to morphological, physiological and biochemical and the 16S rDNA gene sequence was blasted from www. ncbi. nlm. nih. gov/genbank/. As a result, it was bacterial of Rhodobacter sp.. It was adapted to plant wastewater of slanting salt and phenol. It was able to biodegradation para-tertiary butyl phenol in the wastewater. In this study, this discovers laying foundation is going to contribute the microorganism of solving the waste water share.