RP-HPLC法测定人血清中舒芬太尼的浓度

Determination of Sufentanil Concentration in Human Serum by RP-HPLC

目的:建立测定人血清中舒芬太尼浓度的简便方法。方法:血清中舒芬太尼用乙酸乙酯-石油醚(4:1)单步提取后采用反相高效液相色谱法进行测定,其中色谱柱为Symmetry C18,流动相为乙腈-0.02mol.L-1醋酸铵-盐酸(pH4.3)(45:55),检测波长为230nm,柱温为室温,内标为艾司唑仑。结果:舒芬太尼血药浓度在0.025～4.000μg.mL-1范围内线性关系良好(r=0.9999);日内、日间RSD分别为3.49%～4.51%、4.67%～5.99%,平均回收率为101.92%～107.04%。结论:本方法提取步骤少,方法简单、快速、专一性强、准确,适合人血清中舒芬太尼的浓度监测。

OBJECTIVE： To establish the method for the determination of sufentanil concentration in human serum. METH- ODS： Sufentanil was extracted from serum by ethyl ethanoate-petroleum ether（4：1） and determined by RP-HPLC. The determination was performed on Symmetry C18 column with mobile phase consisted of acetonitrile-0.02 mol. L ^-1 NH4Ac-HCL （pH4.3）（45： 55）. The detection wavelength was set at 230 nm and the column temperature was room temperature. Estazolam was used as internal standard. RESULTS： The linear range of sufentanil was 0.025-4.000μg·mL^-1（r=0.999 9） with intra-day and inter-day RSDs of 3.49% -4.51% and 4.67% -5.99%. Average recoveries were 101.92% -107.04%. CONCLUSION： The method is simple, rapid, accurate and specific with few steps. It is suitable for drug concentration monitoring of sufentanil in human serum.