胶原-羟基磷灰石人工骨与胶原膜引导牙周组织再生的动物实验研究

Animal Experiment of Periodontal Defect Reconstruction Using Collagen-hydroxyapatite Artificial Bone Plus Collagen Membrane

目的:将胶原-羟基磷灰石人工骨与胶原膜联合应用于修复牙周缺损的动物实验,探讨其用于引导牙周组织再生的可行性。方法:人工构建4只成年Beagle犬下颌后牙区牙周缺损模型,分别随机采用:胶原-羟基磷灰石人工骨/胶原膜、胶原-羟基磷灰石人工骨、空白对照治疗,每组8颗牙,12周后处死动物,进行组织学观察并测量新生组织高度。结果:与单纯植入胶原-羟基磷灰石人工骨组相比,胶原-羟基磷灰石人工骨/胶原膜组获得了更多的新附着,表现为有较多的新生牙槽骨、新生牙周膜和新生牙骨质样组织生长,2组之间新生组织差异有显著性(P<0.05)。结论:胶原-羟基磷灰石人工骨与胶原膜联合运用修复牙周牙槽骨缺损引导牙周组织再生的效果优于单纯植入人工骨。

Objective： To investigate the possibility of using collagen-hydroxyapatite artificial bone plus collagen membrane in the reconstruction of periodontal defect.Methods： Four beagle dogs were constructed periodontal defect in mandibular premolars or molars.24 teeth were divided into three groups randomly.They were collagen-hydroxyapatite artificial bone plus collagen membrane,collagen-hydroxyapatite artificial bone and blank control.The dogs were sacrificed 12 weeks post-surgery,and healing was evaluated histologically.Results： Periodontal defects implanted with collagen-hydroxyapatite artificial bone plus collagen membrane got more neonatal alveolar bone（P0.05）,periodontal membrane（P0.05） and cementum-like tissue（P0.05） than the group with collagen-hydroxyapatite artificial bone only.Conclusion： Collagen-hydroxyapatite artificial bone plus collagen membrane is better than artificial bone only in periodontal regeneration.