密蒙花提取物滴眼剂对实验性干眼症大鼠泪腺组织雄激素受体数量的影响

Effects of Extract of Buddleja officinalis Eye Drops on Androgen Receptors of Lacrimal Gland Cells of Castrated Rats with Dry Eye

目的观察密蒙花提取物滴眼剂对去势所致干眼症雄鼠基础泪液分泌量、泪膜稳定性及泪腺中雄激素受体(AR)表达的影响,探讨密蒙花提取物滴眼剂抗雄激素水平下降所致干眼症的作用机制。方法将45只Wistar雄性大鼠随机分为空白组、模型组、密蒙花提取物滴眼剂治疗组(治疗组),每组又分为饲养1、2、3个月的3个亚组,共9组,每组5只,模型组及治疗组行去势术建立动物模型,治疗组以密蒙花提取物滴眼剂连续滴眼治疗1个月,对大鼠行Schirmer Ⅰ试验(SIT)、测量泪膜破裂时间(BUT),采用流式细胞仪检测泪腺中AR的表达。结果模型组1、2、3个月亚组SIT值、BUT及AR表达低于同期空白组(P<0.01);治疗组与模型组间比较以3个月的亚组为例,给药后,治疗组SIT值(12.667±5.221)mm,明显高于模型组(2.676±1.987)mm;治疗组BUT(11.758±4.415)s,明显长于模型组(4.667±2.108)s;治疗组AR阳性表达率(49.33±3.44)%,明显高于模型组(33.32±7.12)%(P<0.01)。结论密蒙花提取物滴眼剂中主要成分为黄酮类物质,可显著抑制雄激素水平降低后大鼠干眼症的发生,其作用机制可能与黄酮类物质结构与雄激素类似相关。

Objective To observe the effects of the extract of Buddleja officinalis eye drops （EBOED） on basic tears secretory volume, tear film stability, and expressions of androgen receptors （AR） in castrated rats with dry eye, and to investigate the mechanism of EBOED on dry eye caused by decreased anti-androgen levels. Meth- ods Forty-five male Wistar rats were randomly divided into the blank group, the model group, and the treatment group （treated by EBOED）, respectively. Rats in each group were further divided into three sub-groups （fed for one month, two months, and three months, respectively）. There were totally nine groups, with five in each. The dry eye model was established with orchiectomy of rats in the model group and the treatment group. EBOED was given to rats in the treatment group for one successive month. Schirmer I test （SIT） and breakup time of tear film （BUT） were determined in all experimental rats. Expressions of AR was analyzed by flow cytometer. Results Ths SIT value, BUT, and AR positive rate in the model group at the 1st, 2nd, 3rd month were lower than those in the blank group of the same time points （P 〈0.01 ）. There was statistical difference in SIT value, BUT, and AR positive rate between the model group and the treament group at the three time points （ P 〈 0. 01 ）. Take the threemonth subgroup as an example, the SIT value in the treatment group was （12. 667 ±5. 221 ） mm, obviously higher than that in the model group （2. 676±1. 987） mm. The BUT in the treatment group was （11. 758 ±4. 415） s, obviously longer than that of the model group （4. 667 ±2. 108）s. The AR positive rate in the treatment group was 49. 33% ±3. 44%, obviously higher than that of the model group （33. 32% ±7. 12%, all P 〈0. 01 ）. Conclusions The main components of EBOED was the flavonoids which could significantly inhibit the occurrence of dry eye in rats with decreased androgen levels. Its mechanism might possibly be similar to androgen.